| Title |
Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis
|
|---|---|
| Published in |
Proteome Science, September 2015
|
| DOI | 10.1186/s12953-015-0079-0 |
| Pubmed ID | |
| Authors |
Michal Mikula, Tymon Rubel, Jakub Karczmarski, Malgorzata Statkiewicz, Karol Bomsztyk, Jerzy Ostrowski |
| Abstract |
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centrifugations. As an alternative, we present Matrix-IP, beads-free microplate-based platform with surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that are laboratory-fabricated with UV-C light and then protein A/G coated prior to IP reaction. We demonstrate application of Matrix-IP platform in MS analysis of heterogeneous nuclear ribonucleoprotein K (hnRNP K) interactome and PTMs. Matrix-IP is time-saving, easy to use high throughput method adaptable for low sample amounts and automation. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Unknown | 38 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 38% |
| Researcher | 8 | 21% |
| Student > Bachelor | 6 | 15% |
| Student > Master | 3 | 8% |
| Professor | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 3 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 38% |
| Biochemistry, Genetics and Molecular Biology | 10 | 26% |
| Medicine and Dentistry | 3 | 8% |
| Neuroscience | 3 | 8% |
| Immunology and Microbiology | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 4 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2015.
All research outputs
#18,425,370
of 22,826,360 outputs
Outputs from Proteome Science
#133
of 192 outputs
Outputs of similar age
#192,636
of 267,079 outputs
Outputs of similar age from Proteome Science
#3
of 4 outputs
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