| Title |
Detecting a wide range of environmental contaminants in human blood samples—combining QuEChERS with LC-MS and GC-MS methods
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, July 2015
|
| DOI | 10.1007/s00216-015-8857-1 |
| Pubmed ID | |
| Authors |
Merle M. Plassmann, Magdalena Schmidt, Werner Brack, Martin Krauss |
| Abstract |
Exposure to environmental pollution and consumer products may result in an uptake of chemicals into human tissues. Several studies have reported the presence of diverse environmental contaminants in human blood samples. However, previously developed multi-target methods for the analysis of human blood include a fairly limited amount of compounds stemming from one or two related compound groups. Thus, the sample preparation method QuEChERS (quick easy cheap effective rugged and safe) was tested for the extraction of 64 analytes covering a broad compound domain followed by detection using liquid and gas chromatography coupled to mass spectrometry (LC- and GC-MS). Forty-seven analytes showed absolute recoveries above 70 % in the first QuEChERS step, being a simple liquid-liquid extraction (LLE) using acetonitrile and salt. The second QuEChERS step, being a dispersive solid phase extraction, did not result in an overall improvement of recoveries or removal of background signals. Using solely the LLE step, eight analytes could subsequently be detected in human blood samples from the German Environmental Specimen Bank. Using a LC-multiple reaction monitoring (MRM) method with a triple quadrupole instrument, better recoveries were achieved than with an older LC-high-resolution (HR) MS full scan orbitrap instrument, which required a higher concentration factor of the extracts. However, the application of HRMS full scan methods could be used for the detection of additional compounds retrospectively. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Unknown | 71 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 26% |
| Student > Master | 10 | 14% |
| Researcher | 9 | 13% |
| Student > Bachelor | 6 | 8% |
| Student > Doctoral Student | 3 | 4% |
| Other | 11 | 15% |
| Unknown | 14 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 19 | 26% |
| Environmental Science | 15 | 21% |
| Pharmacology, Toxicology and Pharmaceutical Science | 9 | 13% |
| Agricultural and Biological Sciences | 3 | 4% |
| Biochemistry, Genetics and Molecular Biology | 1 | 1% |
| Other | 6 | 8% |
| Unknown | 19 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2015.
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#22,759,802
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Outputs from Analytical & Bioanalytical Chemistry
#7,542
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#234,917
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#79
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