| Title |
Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, July 2018
|
| DOI | 10.1186/s12906-018-2270-1 |
| Pubmed ID | |
| Authors |
Chin Theng Ng, Lai Yen Fong, Jun Jie Tan, Nor Fadilah Rajab, Faridah Abas, Khozirah Shaari, Kok Meng Chan, Fariza Juliana, Yoke Keong Yong |
| Abstract |
Clinacanthus nutans (Burm. f.) Lindau. has traditionally been using in South East Asia countries to manage cancer. However, scientific evidence is generally lacking to support this traditional claim. This study aims to investigate the in vitro, ex-vivo and in vivo effects of C. nutans extracts on angiogenesis. C. nutans leaves was extracted with 50-100% ethanol or deionised water at 1% (w/v). Human umbilical veins endothelial cell (HUVEC) proliferation was examined using MTT assay. The in vitro anti-angiogenic effects of C. nutans were assessed using wound scratch, tube formation and transwell migration assays. The VEGF levels secreted by human oral squamous cell carcinoma (HSC-4) cell and HUVEC permeability were also measured. Besides, the rat aortic ring and chick embryo chorioallantoic membrane (CAM) assays, representing ex vivo and in vivo models, respectively, were performed. The MTT assay revealed that water extract of C. nutans leaves exhibited the highest activity, compared to the ethanol extracts. Therefore, the water extract was chosen for subsequent experiments. C. nutans leaf extract significantly suppressed endothelial cell proliferation and migration in both absence and presence of VEGF. However, the water extract failed to suppress HUVEC transmigration, differentiation and permeability. C. nutans water extract also did not suppress HSC-4 cell-induced VEGF production. Importantly, C. nutans water extract significantly abolished the sprouting of vessels in aortic rings as well as in chick embryo CAM. In conclusion, these findings reveal potential anti-angiogenic effects of C. nutans, providing new evidence for its potential application as an anti-angiogenic agent. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 65 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 15% |
| Lecturer | 7 | 11% |
| Student > Bachelor | 7 | 11% |
| Other | 4 | 6% |
| Student > Master | 4 | 6% |
| Other | 13 | 20% |
| Unknown | 20 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 14% |
| Medicine and Dentistry | 6 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 8% |
| Chemistry | 4 | 6% |
| Agricultural and Biological Sciences | 3 | 5% |
| Other | 10 | 15% |
| Unknown | 28 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2018.
All research outputs
#20,525,274
of 23,094,276 outputs
Outputs from BMC Complementary Medicine and Therapies
#3,001
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Outputs of similar age
#287,067
of 327,716 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#54
of 73 outputs
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