↓ Skip to main content

Seminal plasma protein profiles of ejaculates obtained by internal artificial vagina and electroejaculation in Brahman bulls

Overview of attention for article published in Animal Reproduction Science, August 2015
Altmetric Badge

Mentioned by

facebook
1 Facebook page

Citations

dimensions_citation
19 Dimensions

Readers on

mendeley
60 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Seminal plasma protein profiles of ejaculates obtained by internal artificial vagina and electroejaculation in Brahman bulls
Published in
Animal Reproduction Science, August 2015
DOI 10.1016/j.anireprosci.2015.07.015
Pubmed ID
Authors

J.P.A. Rego, A.A. Moura, A.S. Nouwens, M.R. McGowan, G.B. Boe-Hansen

Abstract

The present study was conducted to investigate if differences exist in the seminal plasma protein profile from mature Brahman bulls using two methods of semen collection: internal artificial vagina (IAV) and electroejaculation (EEJ). Semen was collected four times from three bulls on the same day and parameters were assessed immediately post-collection. Seminal plasma proteins were evaluated by 2-D fluorescence difference gel electrophoresis and identified by mass spectrometry. Semen volume was greater (P<0.05) for EEJ (4.6±0.35mL) than for IAV (1.86±0.24mL) but sperm concentration was greater in IAV (1505±189×10(6)sperm/mL) than in EEJ samples (344±87×10(6)sperm/mL). Sperm motility and the percentage of normal sperm were not different between treatments. Total concentration of seminal plasma proteins was greater for samples collected by IAV as compared to EEJ (19.3±0.9 compared with 13.0±1.8mg/mL, P<0.05; respectively). Based on 2-D gels, 22 spots had a greater volume (P<0.05) in gels derived from IAV samples, corresponding to 21 proteins identified as transferrin, albumin, epididymal secretory glutathione peroxidase, among others. Thirty-three spots, corresponding to 26 proteins, had a greater volume (P<0.05) in gels derived from EEJ samples. These proteins were identified as spermadhesin-1, Bovine Sperm Protin 1, 3 and 5 isoforms, angiogenin-1, alpha-1B-glycoprotein, clusterin, nucleobindin-1, cathepsins, spermadhesin Z13, annexins, among others. Thus, proteins in greater amounts in samples obtained by IAV and EEJ were mainly of epididymal origin and accessory sex glands, respectively.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 60 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 17%
Student > Ph. D. Student 7 12%
Student > Bachelor 7 12%
Student > Doctoral Student 4 7%
Researcher 4 7%
Other 13 22%
Unknown 15 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 32%
Veterinary Science and Veterinary Medicine 10 17%
Biochemistry, Genetics and Molecular Biology 6 10%
Medicine and Dentistry 2 3%
Environmental Science 1 2%
Other 2 3%
Unknown 20 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 August 2015.
All research outputs
#22,778,604
of 25,394,764 outputs
Outputs from Animal Reproduction Science
#826
of 1,133 outputs
Outputs of similar age
#236,203
of 275,907 outputs
Outputs of similar age from Animal Reproduction Science
#9
of 18 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,133 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,907 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.