| Title |
Interactomic study on interaction between lipid droplets and mitochondria
|
|---|---|
| Published in |
Protein & Cell, July 2011
|
| DOI | 10.1007/s13238-011-1061-y |
| Pubmed ID | |
| Authors |
Jing Pu, Cheol Woong Ha, Shuyan Zhang, Jong Pil Jung, Won-Ki Huh, Pingsheng Liu |
| Abstract |
An increasing body of evidence shows that the lipid droplet, a neutral lipid storage organelle, plays a role in lipid metabolism and energy homeostasis through its interaction with mitochondria. However, the cellular functions and molecular mechanisms of the interaction remain ambiguous. Here we present data from transmission electron microscopy, fluorescence imaging, and reconstitution assays, demonstrating that lipid droplets physically contact mitochondria in vivo and in vitro. Using a bimolecular fluorescence complementation assay in Saccharomyces cerevisiae, we generated an interactomic map of protein-protein contacts of lipid droplets with mitochondria and peroxisomes. The lipid droplet proteins Erg6 and Pet10 were found to be involved in 75% of the interactions detected. Interestingly, interactions between 3 pairs of lipid metabolic enzymes were detected. Collectively, these data demonstrate that lipid droplets make physical contacts with mitochondria and peroxisomes, and reveal specific molecular interactions that suggest active participation of lipid droplets in lipid metabolism in yeast. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 164 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Israel | 1 | <1% |
| Sweden | 1 | <1% |
| South Africa | 1 | <1% |
| Unknown | 161 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 44 | 27% |
| Researcher | 27 | 16% |
| Student > Master | 22 | 13% |
| Student > Bachelor | 15 | 9% |
| Student > Doctoral Student | 9 | 5% |
| Other | 26 | 16% |
| Unknown | 21 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 57 | 35% |
| Biochemistry, Genetics and Molecular Biology | 45 | 27% |
| Neuroscience | 6 | 4% |
| Medicine and Dentistry | 6 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 3% |
| Other | 17 | 10% |
| Unknown | 28 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 July 2013.
All research outputs
#17,651,093
of 22,656,971 outputs
Outputs from Protein & Cell
#538
of 736 outputs
Outputs of similar age
#95,966
of 116,710 outputs
Outputs of similar age from Protein & Cell
#5
of 7 outputs
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