| Title |
RETRACTED ARTICLE: Upregulation of miR-300 and downregulation of miR-125b act as potential predictor biomarkers in progression, metastasis, and poor prognosis of osteosarcoma
|
|---|---|
| Published in |
Tumor Biology, September 2015
|
| DOI | 10.1007/s13277-015-4000-3 |
| Pubmed ID | |
| Authors |
Seyyed Hasan Karbasy, Afshin Taheriazam, Alireza Mirghasemi, Farnoush Sedaghati, Mohammadreza Shakeri, Emad Yahaghi, Reza Bahador |
| Abstract |
MiRNAs play crucial roles in development of cancer. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) are poorly understood. This study is aimed to investigate the clinical importance of miR-125b and miR-300 expression in OS tissue samples using real-time PCR.Total RNA was purified from samples and noncancerous bone tissues and then real-time PCR was used to evaluate the expression rate of microRNAs. Furthermore, the association of miR-125b and MiR-300 level with pathological factors and prognosis were evaluated. Our results suggested that miR-125b expression was significantly downregulated in osteosarcoma bone tissue compared with noncancerous bone tissues. On the other hand, miR-300 expression was significantly upregulated in OS bone tissue than noncancerous bone tissues.Our findings suggested that a lower expression level of miR-125b was linked to advanced clinical stage (P = 0.005), metastasis (P = 0.004), and a large size of tumor (P = 0.023). In addition, a higher expression level of miR-300 was related to advanced tumor, node, and metastasis (TNM) stage (P = 0.002), metastasis (P = 0.001), and a large size of tumor (P = 0.038). The lower expression and higher expression of mentioned microRNAs was significantly linked to shorter overall survival according to the Kaplan-Meier survival and log-rank analysis (log-rank test P = 0.026; P = 0.017).Moreover, lower expression of miR-125b (P = 0.001), a decreased expression level of miR-300 (P = 0.004), TNM stage (P = 0. 0.004; P = 0.003), metastasis (P = 0.027; P = 0.001), and a large tumor size (P = 0.006; P = 0.025) were independent prognostic markers of overall survival of patients according to the multivariate Cox proportional hazards model. In conclusion, our results indicated that downregulation of miR-125b and miR-300 was associated with progression of OS and both of them may act as tumor suppressor in OS. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 29% |
| Researcher | 2 | 29% |
| Student > Doctoral Student | 1 | 14% |
| Student > Master | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 57% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Linguistics | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2015.
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#20,290,425
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#1,834
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Outputs of similar age from Tumor Biology
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