| Title |
Immunoassay and amperometric biosensor approaches for the detection of deltamethrin in seawater
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, July 2018
|
| DOI | 10.1007/s00216-018-1209-1 |
| Pubmed ID | |
| Authors |
Philipp Fruhmann, Ana Sanchis, Lisa Mayerhuber, Tünde Vanka, Christoph Kleber, J.-Pablo Salvador, M.-Pilar Marco |
| Abstract |
The study of an enzyme-linked immunosorbent assay (ELISA) and an amperometric biosensor for the detection of the pyrethroid deltamethrin in seawater is reported. The preparation of specific polyclonal antibodies is addressed using two immunizing haptens based on deltamethrin and cypermethrin compounds, with a spacer arm placed at the cyano residue in the pyrethroid structure. Different conjugates based on bovine serum albumin and aminodextran are prepared depending on the lipophilic profile of the competitor haptens studied. A reproducible and sensitive indirect competitive ELISA is developed, reaching a limit of detection of 1.2 ± 0.04 μg L-1 and an IC50 value of 21.4 ± 0.3 μg L-1 (both n = 3). For validation of the assays described, artificial seawater samples fortified with deltamethrin are analyzed. For the ELISA assay, these accuracy studies reported a slope of 0.904. An amperometric immunosensor is developed using the same immunoreagents and achieving a comparable detectability in terms of LOD of 4.7 μg L-1, measuring seawater without any pretreatment. These results suggest that both techniques can be used as rapid and simple analytical methods for deltamethrin quantification in seawater samples, which are great candidates for initial environmental screening programs. Graphical abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 32% |
| Student > Bachelor | 5 | 14% |
| Student > Master | 4 | 11% |
| Student > Ph. D. Student | 4 | 11% |
| Professor | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 7 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 13 | 35% |
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Agricultural and Biological Sciences | 3 | 8% |
| Physics and Astronomy | 3 | 8% |
| Chemical Engineering | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 11 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 March 2019.
All research outputs
#16,835,337
of 25,728,855 outputs
Outputs from Analytical & Bioanalytical Chemistry
#5,288
of 9,730 outputs
Outputs of similar age
#209,029
of 341,660 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#75
of 178 outputs
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