| Title |
Regulation of Unbalanced Redox Homeostasis Induced by the Expression of Wild-Type HIV-1 Viral Protein R (NL4-3Vpr) in Fission Yeast
|
|---|---|
| Published in |
Biologia Futura, December 2015
|
| DOI | 10.1556/018.66.2015.3.8 |
| Pubmed ID | |
| Authors |
Zoltán Gazdag, Timea Stromájer-rácz, Joseph Belagyi, Richard Y. Zhao, Robert T. Elder, Eszter Virág, Miklós Pesti |
| Abstract |
The wild-type viral protein R (Vpr) of human immunodeficiency virus type 1 exerts multiple effects on cellular activities during infection, including the induction of cell cycle G2 arrest and the death of human cells and cells of the fission yeast Schizosaccharomyces pombe. In this study, wild-type Vpr (NL4-3Vpr) integrated as a single copy gene in S. pombe chromosome was used to investigate the molecular impact of Vpr on cellular oxidative stress. NL4-3Vpr triggered an atypical response in early (14-h), and a wellregulated oxidative stress response in late (35-h) log-phase cultures. Specifically, NL4-3Vpr expression induced oxidative stress in the 14-h cultures leading, to decreased levels of superoxide anion (O2(·-)), hydroxyl radical (·OH) and glutathione (GSH), and significantly decreased activities of catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and glutathione S-transferase. In the 35-h cultures, elevated levels of O2(·-) and peroxides were accompanied by increased activities of most antioxidant enzymes, suggesting that the Vpr-induced unbalanced redox state of the cells might contribute to the adverse effects in HIV-infected patients. |
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| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Professor | 1 | 9% |
| Student > Bachelor | 1 | 9% |
| Student > Master | 1 | 9% |
| Student > Postgraduate | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 4 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Immunology and Microbiology | 2 | 18% |
| Medicine and Dentistry | 2 | 18% |
| Social Sciences | 1 | 9% |
| Unknown | 4 | 36% |