| Title |
Antimicrobial resistance and virulence factor gene profiles of Enterococcus spp. isolates from wild Arctocephalus australis (South American fur seal) and Arctocephalus tropicalis (Subantarctic fur seal)
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, September 2015
|
| DOI | 10.1007/s11274-015-1938-7 |
| Pubmed ID | |
| Authors |
Naiara Aguiar Santestevan, Dejoara de Angelis Zvoboda, Janira Prichula, Rebeca Inhoque Pereira, Guilherme Raffo Wachholz, Leonardo Almansa Cardoso, Tiane Martin de Moura, Aline Weber Medeiros, Derek Blaese de Amorin, Maurício Tavares, Pedro Alves d’Azevedo, Ana Claudia Franco, Jeverson Frazzon, Ana Paula Guedes Frazzon |
| Abstract |
Enterococci are natural inhabitants of the gastrointestinal tracts in humans and animals. Epidemiological data suggest that enterococci are important reservoirs of antimicrobial resistant genes that may be transmitted from other bacterial species The aim of this study was to investigate the species composition, antimicrobial resistance and virulence genes in enterococci recovered from fecal samples of wild Arctocephalus australis and A. tropicalis found dead along the South Coast of Brazil. From a total of 43 wild fur seals, eleven were selected for this study. Phenotypic and genotypic characterizations were used to classify Enterococcus species. Strains were tested for susceptibility to 10 antibiotics, presence of ace, gelE, asa, cylA, tet(L), tet(M) and erm(B) genes by PCR, and genetic variability using RAPD-PCR. Among the 50 enterococci isolated, 40 % were Enterococcus faecalis, 40 % E. hirae, 12 % E. casseliflavus and 8 % other enterococcal species. Resistance profiles were observed to erythromycin, nitrofurantoin, tetracycline, norfloxacin and ciprofloxacin. The prevalence of virulence genes was ace (68 %), gelE (54 %), asa (22 %) and cylA (4 %). In erythromycin- and tetracycline strains, erm(B) and tet(M) were detected, respectively. The RAPD-PCR demonstrated a close phylogenetic relationship between the enterococci isolated from A. australis and A. tropicalis. In conclusion, different enterococcus species showing antimicrobial resistance and virulence determinates were isolated from fecal samples of fur seals. Antibiotic resistant strains in these animals could be related within food chain and aquatic pollutants or linked to environmental resistome, and demonstrates the potential importance of these animals as reservoirs and disseminators of such determinants in marine environmental. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 74 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 16% |
| Student > Master | 11 | 15% |
| Student > Bachelor | 10 | 14% |
| Student > Ph. D. Student | 7 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Other | 8 | 11% |
| Unknown | 22 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 28% |
| Biochemistry, Genetics and Molecular Biology | 8 | 11% |
| Medicine and Dentistry | 7 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
| Veterinary Science and Veterinary Medicine | 3 | 4% |
| Other | 8 | 11% |
| Unknown | 24 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2015.
All research outputs
#16,223,992
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#990
of 1,757 outputs
Outputs of similar age
#160,648
of 271,095 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#14
of 34 outputs
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