Title |
Grapevine virus T is relatively widespread in Slovakia and Czech Republic and genetically diverse
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Published in |
Virus Genes, July 2018
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DOI | 10.1007/s11262-018-1587-7 |
Pubmed ID | |
Authors |
Miroslav Glasa, Lukáš Predajňa, Nina Sihelská, Katarína Šoltys, Ana Belén Ruiz-García, Antonio Olmos, Thierry Wetzel, Sead Sabanadzovic |
Abstract |
A recently described putative foveavirus, grapevine virus T (GVT), was detected in a Slovak grapevine accession (SK704) using high-throughput sequencing, prompting further studies. Full-length genome sequence of isolate GVT-SK704 was determined. Analyses revealed 86.1% nucleotide identity with the Italian GVT isolate, currently the only available nearly complete sequence of GVT in GenBank. A virus-specific RT-PCR assay was developed, which enabled a survey of GVT incidence in grapevine samples from Slovakia and Czech Republic. Unexpectedly, GVT was present in ~ 30% of tested samples. Analysis of complete CP gene sequences of 20 Slovak and Czech GVT isolates detected in the survey revealed relatively high intra-species variability (up to 11.2% nucleotide divergence), suggesting multiple introductions from different sources, possibly over an extended period of time. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 33% |
Student > Ph. D. Student | 2 | 13% |
Student > Bachelor | 2 | 13% |
Student > Master | 1 | 7% |
Unknown | 5 | 33% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 6 | 40% |
Biochemistry, Genetics and Molecular Biology | 3 | 20% |
Immunology and Microbiology | 1 | 7% |
Unknown | 5 | 33% |