| Title |
LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53
|
|---|---|
| Published in |
Cellular & Molecular Biology Letters, July 2018
|
| DOI | 10.1186/s11658-018-0099-8 |
| Pubmed ID | |
| Authors |
Shaoxian Liu, Junyi Yue, Wei Du, Jian Han, Weidong Zhang |
| Abstract |
Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown. We knocked down and overexpressed LAMP3 in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosis-associated genes to identify the downstream gene(s) of LAMP3. Knockdown of LAMP3 significantly inhibited OS cell viability and promoted apoptosis. TP53, which is involved in the apoptosis pathway, was found to be highly upregulated after knockdown of LAMP3. Overexpression of LAMP3 significantly increased cell viability and abrogated apoptosis. Importantly, subsequent knockdown of TP53 partially suppressed the increased OS cell apoptosis induced by the inhibition of LAMP3, suggesting that TP53 is a key functional downstream gene of LAMP3. Our findings suggest that LAMP3 promotes OS cell viability and survival by regulating TP53 expression. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 29% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Researcher | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 43% |
| Agricultural and Biological Sciences | 2 | 29% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Unknown | 1 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2018.
All research outputs
#18,641,800
of 23,094,276 outputs
Outputs from Cellular & Molecular Biology Letters
#255
of 488 outputs
Outputs of similar age
#252,271
of 326,767 outputs
Outputs of similar age from Cellular & Molecular Biology Letters
#7
of 15 outputs
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