| Title |
Identification and characterisation of short chain rhamnolipid production in a previously uninvestigated, non-pathogenic marine pseudomonad
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, July 2018
|
| DOI | 10.1007/s00253-018-9202-3 |
| Pubmed ID | |
| Authors |
Matthew S. Twigg, L. Tripathi, A. Zompra, K. Salek, V. U. Irorere, T. Gutierrez, G. A. Spyroulias, R. Marchant, I. M. Banat |
| Abstract |
This study aimed to identify and characterise biosurfactant compounds produced by bacteria associated with a marine eukaryotic phytoplankton bloom. One strain, designated MCTG214(3b1), was isolated by enrichment with polycyclic aromatic hydrocarbons and based on 16S rDNA, and gyrB sequencing was found to belong to the genus Pseudomonas, however not related to P. aeruginosa. Cell-free supernatant samples of strain MCTG214(3b1) at stationary phase showed significant reductions in surface tension. HPLC-MS and NMR analysis of these samples indicated the presence of five different rhamnolipid (RL) congeners. Di-rhamnolipids accounted for 87% relative abundance and all congeners possessed fatty acid moieties consisting of 8-12 carbons. PCR screening of strain MCTG214(3b1) DNA revealed homologues to the P. aeruginosa RL synthesis genes rhlA and rhlB; however, no rhlC homologue was identified. Using the Galleria mellonella larvae model, strain MCTG214(3b1) was demonstrated to be far less pathogenic than P. aeruginosa. This study identifies for the first time a significantly high level of synthesis of short chain di-rhamnolipids by a non-pathogenic marine Pseudomonas species. We postulate that RL synthesis in Pseudomonas sp. MCTG214(3b1) is carried out by enzymes expressed from rhlA/B homologues similar to those of P. aeruginosa; however, a lack of rhlC potentially indicates the presence of a second novel rhamnosyltransferase responsible for the di-rhamnolipid congeners identified by HPLC-MS. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 89 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 17% |
| Student > Ph. D. Student | 13 | 15% |
| Student > Master | 12 | 13% |
| Student > Bachelor | 8 | 9% |
| Student > Doctoral Student | 3 | 3% |
| Other | 7 | 8% |
| Unknown | 31 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 21% |
| Agricultural and Biological Sciences | 13 | 15% |
| Environmental Science | 6 | 7% |
| Immunology and Microbiology | 5 | 6% |
| Chemical Engineering | 4 | 4% |
| Other | 9 | 10% |
| Unknown | 33 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2018.
All research outputs
#18,922,529
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#6,246
of 8,034 outputs
Outputs of similar age
#240,715
of 330,161 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#89
of 156 outputs
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