| Title |
An enzyme-free homogenous electrochemical assay for sensitive detection of the plasmid-mediated colistin resistance gene mcr-1
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, May 2018
|
| DOI | 10.1007/s00216-018-1130-7 |
| Pubmed ID | |
| Authors |
Bo Li, Zhixin Chai, Xiaohui Yan, Chunchen Liu, Bo Situ, Ye Zhang, Weilun Pan, Shihua Luo, Jianhua Liu, Lei Zheng |
| Abstract |
Antibiotic resistance associated with the mcr-1 gene of Gram-negative bacteria, which confers resistance to drugs of last resort and has the potential to spread via plasmids, is one of the most pressing issues facing global health today. Point-of-care testing for the mcr-1 gene is needed to aid in the identification of colistin resistance in the field and to control its horizontal transmission. Here, we report the successful development of an enzyme-free homogenous electrochemical strategy for sensitive detection of the antibiotic resistance gene mcr-1 using the hybridization chain reaction and mcr-1-specific toehold probe. The long double-stranded DNA polymer produced using this strategy could be detected by assessing the diffusion of methylene blue towards the surface of a screen-printed gold electrode. Under optimized conditions, a linear relationship was observed between the variation of peak current and the natural logarithm of the mcr-1 gene concentration in the range of 1 nM to 1 μM with a detection limit of 0.78 nM (S/N = 3). This enzyme-free, isothermal platform is a rapid, portable, disposable, and sensitive method for detection of plasmid-mediated colistin resistance. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 11% |
| Student > Master | 2 | 11% |
| Student > Bachelor | 1 | 5% |
| Lecturer > Senior Lecturer | 1 | 5% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 11 | 58% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 11% |
| Chemistry | 2 | 11% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Chemical Engineering | 1 | 5% |
| Environmental Science | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 10 | 53% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2018.
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#22,767,715
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Outputs from Analytical & Bioanalytical Chemistry
#7,543
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Outputs of similar age
#302,081
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#132
of 187 outputs
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