| Title |
Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
|
|---|---|
| Published in |
Virology Journal, July 2018
|
| DOI | 10.1186/s12985-018-1015-6 |
| Pubmed ID | |
| Authors |
Mariana Kikuti, Laura B. Tauro, Patrícia S. S. Moreira, Gúbio S. Campos, Igor A. D. Paploski, Scott C. Weaver, Mitermayer G. Reis, Uriel Kitron, Guilherme S. Ribeiro |
| Abstract |
Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay (ELISA) kits. We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics in Brazil (2009-2011 and 2013, respectively) to determine specificity. The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors' sera. The ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue (92.3%; 12/13), compared to secondary dengue (7.1%; 3/42). In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 18% |
| Ecuador | 1 | 9% |
| Kenya | 1 | 9% |
| Brazil | 1 | 9% |
| Switzerland | 1 | 9% |
| Unknown | 5 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 64% |
| Science communicators (journalists, bloggers, editors) | 3 | 27% |
| Scientists | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 129 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 129 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 23 | 18% |
| Researcher | 21 | 16% |
| Student > Bachelor | 14 | 11% |
| Student > Ph. D. Student | 11 | 9% |
| Other | 7 | 5% |
| Other | 22 | 17% |
| Unknown | 31 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 22 | 17% |
| Biochemistry, Genetics and Molecular Biology | 19 | 15% |
| Immunology and Microbiology | 16 | 12% |
| Agricultural and Biological Sciences | 10 | 8% |
| Nursing and Health Professions | 4 | 3% |
| Other | 17 | 13% |
| Unknown | 41 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 25. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2020.
All research outputs
#1,556,897
of 25,845,749 outputs
Outputs from Virology Journal
#119
of 3,433 outputs
Outputs of similar age
#31,816
of 342,965 outputs
Outputs of similar age from Virology Journal
#2
of 36 outputs
Altmetric has tracked 25,845,749 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,433 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 19.7. This one has done particularly well, scoring higher than 96% of its peers.
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We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.