| Title |
Binding mechanism of anti-cancer chemotherapeutic drug mitoxantrone to DNA characterized by magnetic tweezers
|
|---|---|
| Published in |
Journal of Nanobiotechnology, July 2018
|
| DOI | 10.1186/s12951-018-0381-y |
| Pubmed ID | |
| Authors |
Dennis Kreft, Ying Wang, Michael Rattay, Katja Toensing, Dario Anselmetti |
| Abstract |
Chemotherapeutic agents (anti-cancer drugs) are small cytostatic or cytotoxic molecules that often bind to double-stranded DNA (dsDNA) resulting in modifications of their structural and nanomechanical properties and thus interfering with the cell proliferation process. We investigated the anthraquinone compound mitoxantrone that is used for treating certain cancer types like leukemia and lymphoma with magnetic tweezers as a single molecule nanosensor. In order to study the association of mitoxantrone with dsDNA, we conducted force-extension and mechanical overwinding experiments with a sensitivity of 10-14 N. Using this method, we were able to estimate an equilibrium constant of association Ka ≈ 1 × 105 M-1 as well as a binding site size of n ≈ 2.5 base pairs for mitoxantrone. An unwinding angle of mitoxantrone-intercalation of ϑ ≈ 16° was determined. Moreover, we observed a complex concentration-dependent bimodal binding behavior, where mitoxantrone associates to dsDNA as an intercalator and groove binder simultaneously at low concentrations and as a mere intercalator at high concentrations. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 20% |
| Student > Bachelor | 5 | 13% |
| Student > Master | 5 | 13% |
| Researcher | 4 | 10% |
| Student > Doctoral Student | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 12 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 28% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 10% |
| Physics and Astronomy | 4 | 10% |
| Engineering | 2 | 5% |
| Chemistry | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 14 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2018.
All research outputs
#15,012,809
of 23,096,849 outputs
Outputs from Journal of Nanobiotechnology
#596
of 1,450 outputs
Outputs of similar age
#197,540
of 327,048 outputs
Outputs of similar age from Journal of Nanobiotechnology
#7
of 11 outputs
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So far Altmetric has tracked 1,450 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 54% of its peers.
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