| Title |
PiggyBac transposon-mediated mutagenesis and application in yeast Komagataella phaffii
|
|---|---|
| Published in |
Biotechnology Techniques, July 2018
|
| DOI | 10.1007/s10529-018-2592-6 |
| Pubmed ID | |
| Authors |
Jinxiang Zhu, Qiaoyun Zhu, Ruiqing Gong, Qin Xu, Menghao Cai, Tianyi Jiang, Xiangshan Zhou, Mian Zhou, Yuanxing Zhang |
| Abstract |
Around one-fourth of the Komagataella phaffii genes encode hypothetical proteins with unknown functions. However, lack of powerful tools for genetic screening in K. phaffii significantly limits the functional analysis of these unknown genes. Transposon mutagenesis has been utilized as an insertional mutagenesis tool in many other organisms and would be extremely valuable if it could be applied in K. phaffii. In this study, we investigated in K. phaffii the transposition activity and efficiency of piggyBac (PB) transposon, a DNA transposon from the cabbage looper moth Trichoplusia ni through the integrated-plasmid system. We also designed a binary-plasmid system which could generate stable mutants. Finally we evaluated the quality of this mutagenesis system by a simple screening for functional genes involved in K. phaffii carbon catabolite repression. Our results demonstrate that PB-mediated mutagenesis could be a feasible and useful tool for functional gene screening in K. phaffii. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 50% |
| Members of the public | 2 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 31% |
| Student > Bachelor | 4 | 10% |
| Student > Ph. D. Student | 4 | 10% |
| Unspecified | 1 | 3% |
| Other | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 15 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 26% |
| Agricultural and Biological Sciences | 7 | 18% |
| Engineering | 2 | 5% |
| Immunology and Microbiology | 2 | 5% |
| Unspecified | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 17 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2019.
All research outputs
#14,920,631
of 25,385,509 outputs
Outputs from Biotechnology Techniques
#2,136
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Outputs of similar age
#175,678
of 339,415 outputs
Outputs of similar age from Biotechnology Techniques
#5
of 9 outputs
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