| Title |
Dynamic nuclear polarization of nucleic acid with endogenously bound manganese
|
|---|---|
| Published in |
Journal of Biomolecular NMR, July 2015
|
| DOI | 10.1007/s10858-015-9972-1 |
| Pubmed ID | |
| Authors |
Patricia Wenk, Monu Kaushik, Diane Richter, Marc Vogel, Beatrix Suess, Björn Corzilius |
| Abstract |
We report the direct dynamic nuclear polarization (DNP) of (13)C nuclei of a uniformly [(13)C,(15)N]-labeled, paramagnetic full-length hammerhead ribozyme (HHRz) complex with Mn(2+) where the enhanced polarization is fully provided by the endogenously bound metal ion and no exogenous polarizing agent is added. A (13)C enhancement factor of ε = 8 was observed by intra-complex DNP at 9.4 T. In contrast, "conventional" indirect and direct DNP experiments were performed using AMUPol as polarizing agent where we obtained a (1)H enhancement factor of ε ≈ 250. Comparison with the diamagnetic (Mg(2+)) HHRz complex shows that the presence of Mn(2+) only marginally influences the (DNP-enhanced) NMR properties of the RNA. Furthermore two-dimensional correlation spectra ((15)N-(13)C and (13)C-(13)C) reveal structural inhomogeneity in the frozen, amorphous state indicating the coexistence of several conformational states. These demonstrations of intra-complex DNP using an endogenous metal ion as well as DNP-enhanced MAS NMR of RNA in general yield important information for the development of new methods in structural biology. |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 87 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 37 | 43% |
| Researcher | 17 | 20% |
| Student > Bachelor | 5 | 6% |
| Student > Master | 5 | 6% |
| Student > Doctoral Student | 3 | 3% |
| Other | 8 | 9% |
| Unknown | 12 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 36 | 41% |
| Biochemistry, Genetics and Molecular Biology | 21 | 24% |
| Agricultural and Biological Sciences | 4 | 5% |
| Physics and Astronomy | 3 | 3% |
| Engineering | 3 | 3% |
| Other | 5 | 6% |
| Unknown | 15 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2015.
All research outputs
#15,346,908
of 22,828,180 outputs
Outputs from Journal of Biomolecular NMR
#381
of 614 outputs
Outputs of similar age
#153,887
of 263,447 outputs
Outputs of similar age from Journal of Biomolecular NMR
#7
of 14 outputs
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