| Title |
Leptin stimulates tissue rat mast cell pro-inflammatory activity and migratory response
|
|---|---|
| Published in |
Inflammation Research, July 2018
|
| DOI | 10.1007/s00011-018-1171-6 |
| Pubmed ID | |
| Authors |
Paulina Żelechowska, Justyna Agier, Sylwia Różalska, Magdalena Wiktorska, Ewa Brzezińska-Błaszczyk |
| Abstract |
The aim of this study was to determine whether leptin, a member of the adipocytokines involved in immune and inflammatory response regulation, may influence some aspects of mast cell biology. Experiments were done in vitro on fully mature tissue rat mast cells isolated from the peritoneal cavity, and leptin was used at concentrations 0.001-100 ng/ml. The effect of leptin on mast cell degranulation (histamine release assay), intracellular Ca2+ level (fluorimetry), pro-inflammatory mediator release (ELISA technique), surface receptor expression (flow cytometry and confocal microscopy), and migration (Boyden microchamber assay) was estimated. Leptin was found to stimulate mast cells to degranulation and histamine release. It induced the intracellular Ca2+ increase, as well. In response to leptin stimulation, mast cells generated and released cysLTs and chemokine CCL3. Leptin-induced upregulation of CYSLTR1 and CYSLTR2 surface expression was observed. Moreover, this adipocytokine stimulated mast cells to migratory response, even in the absence of extracellular matrix (ECM) proteins. Our observations clearly documented that leptin promotes the pro-inflammatory activity of mast cells, and it thereby engages these cells in the inflammatory processes. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 26% |
| Unspecified | 1 | 5% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Student > Ph. D. Student | 1 | 5% |
| Other | 4 | 21% |
| Unknown | 6 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 21% |
| Medicine and Dentistry | 2 | 11% |
| Agricultural and Biological Sciences | 2 | 11% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Unspecified | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 7 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2022.
All research outputs
#19,012,063
of 23,570,677 outputs
Outputs from Inflammation Research
#716
of 980 outputs
Outputs of similar age
#230,812
of 297,506 outputs
Outputs of similar age from Inflammation Research
#9
of 12 outputs
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