| Title |
Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification
|
|---|---|
| Published in |
Virology Journal, November 2011
|
| DOI | 10.1186/1743-422x-8-510 |
| Pubmed ID | |
| Authors |
Hao-tai Chen, Jie Zhang, Yong-sheng Liu, Xiang-tao Liu |
| Abstract |
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Denmark | 1 | 2% |
| South Africa | 1 | 2% |
| Unknown | 44 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 32% |
| Student > Ph. D. Student | 11 | 23% |
| Student > Bachelor | 3 | 6% |
| Student > Doctoral Student | 2 | 4% |
| Professor | 2 | 4% |
| Other | 7 | 15% |
| Unknown | 7 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 34% |
| Veterinary Science and Veterinary Medicine | 9 | 19% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Medicine and Dentistry | 4 | 9% |
| Nursing and Health Professions | 2 | 4% |
| Other | 4 | 9% |
| Unknown | 7 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2012.
All research outputs
#17,651,093
of 22,656,971 outputs
Outputs from Virology Journal
#2,217
of 3,023 outputs
Outputs of similar age
#115,090
of 142,895 outputs
Outputs of similar age from Virology Journal
#73
of 97 outputs
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