| Title |
Development of Patient-Derived Gastric Cancer Organoids from Endoscopic Biopsies and Surgical Tissues
|
|---|---|
| Published in |
Annals of Surgical Oncology, July 2018
|
| DOI | 10.1245/s10434-018-6662-8 |
| Pubmed ID | |
| Authors |
Mei Gao, Miranda Lin, Manisha Rao, Hannah Thompson, Kelsi Hirai, Minsig Choi, Georgios V. Georgakis, Aaron R. Sasson, Juan Carlos Bucobo, Demetri Tzimas, Lionel S. D’Souza, Jonathan M. Buscaglia, James Davis, Kenneth R. Shroyer, Jinyu Li, Scott Powers, Joseph Kim |
| Abstract |
Organoids are three-dimensional in vitro models of human disease developed from benign and malignant gastrointestinal tissues with tremendous potential for personalized medicine applications. We sought to determine whether gastric cancer patient-derived organoids (PDOs) could be safely established from endoscopic biopsies for rapid drug screening. Patients underwent esophagogastroduodenoscopy (EGD) for surveillance or staging and had additional forceps biopsies taken for PDO creation. Cancer tissues from operative specimens were also used to create PDOs. To address potential tumor heterogeneity, we performed low-coverage whole-genome sequencing of endoscopic-derived PDOs with paired surgical PDOs and whole-tumor lysates. The stability of genomic alterations in endoscopic organoids was assessed by next-generation sequencing and nested polymerase chain reaction (PCR) assay. The feasibility and potential accuracy of drug sensitivity screening with endoscopic-derived PDOs were also evaluated. Gastric cancer PDOs (n = 15) were successfully established from EGD forceps biopsies (n = 8) and surgical tissues (n = 7) from five patients with gastric adenocarcinoma. Low-coverage whole-genomic profiling of paired EGD and surgical PDOs along with whole-tumor lysates demonstrated absence of tumor heterogeneity. Nested PCR assay identified similar KRAS alterations in primary tumor and paired organoids. Drug sensitivity testing of endoscopic-derived PDOs displayed standard dose-response curves to current gastric cancer cytotoxic therapies. Our study results demonstrate the feasibility of developing gastric cancer PDOs from EGD biopsies. These results also indicate that endoscopic-derived PDOs are accurate surrogates of the primary tumor and have the potential for drug sensitivity screening and personalized medicine applications. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 87 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 15% |
| Student > Master | 10 | 11% |
| Student > Bachelor | 8 | 9% |
| Researcher | 6 | 7% |
| Student > Doctoral Student | 6 | 7% |
| Other | 13 | 15% |
| Unknown | 31 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 22% |
| Medicine and Dentistry | 15 | 17% |
| Agricultural and Biological Sciences | 9 | 10% |
| Chemical Engineering | 2 | 2% |
| Engineering | 2 | 2% |
| Other | 6 | 7% |
| Unknown | 34 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 May 2020.
All research outputs
#4,590,661
of 23,096,849 outputs
Outputs from Annals of Surgical Oncology
#1,451
of 6,550 outputs
Outputs of similar age
#87,635
of 326,949 outputs
Outputs of similar age from Annals of Surgical Oncology
#59
of 126 outputs
Altmetric has tracked 23,096,849 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 6,550 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 326,949 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.