Title |
NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin
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Published in |
Molecular and Cellular Biochemistry, July 2018
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DOI | 10.1007/s11010-018-3412-y |
Pubmed ID | |
Authors |
Gökhan Kasnak, Eija Könönen, Stina Syrjänen, Mervi Gürsoy, Fares Zeidán-Chuliá, Erhan Firatli, Ulvi K. Gürsoy |
Abstract |
Bacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO). HMK cells were incubated with Pg LPS (1 µl/ml), nicotine (1.54 mM), and 4-NQO (1 µM) for 24 h. Intracellular and extracellular levels of interleukin (IL)-1β, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex® xMAP™ technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction. All tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1β and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels. Pg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 20% |
Professor | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Student > Master | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 0 | 0% |
Unknown | 6 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Psychology | 1 | 7% |
Nursing and Health Professions | 1 | 7% |
Unknown | 9 | 60% |