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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum
|
|---|---|
| Published in |
Nature Communications, September 2015
|
| DOI | 10.1038/ncomms9410 |
| Pubmed ID | |
| Authors |
Sangwoo Kim, Yu-Sin Jang, Sung-Chul Ha, Jae-Woo Ahn, Eun-Jung Kim, Jae Hong Lim, Changhee Cho, Yong Shin Ryu, Sung Kuk Lee, Sang Yup Lee, Kyung-Jin Kim |
| Abstract |
Thiolase is the first enzyme catalysing the condensation of two acetyl-coenzyme A (CoA) molecules to form acetoacetyl-CoA in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel. Here we elucidate the crystal structure of Clostridium acetobutylicum thiolase (CaTHL) in its reduced/oxidized states. CaTHL, unlike those from other aerobic bacteria such as Escherichia coli and Zoogloea ramegera, is regulated by the redox-switch modulation through reversible disulfide bond formation between two catalytic cysteine residues, Cys88 and Cys378. When CaTHL is overexpressed in wild-type C. acetobutylicum, butanol production is reduced due to the disturbance of acidogenic to solventogenic shift. The CaTHL(V77Q/N153Y/A286K) mutant, which is not able to form disulfide bonds, exhibits higher activity than wild-type CaTHL, and enhances butanol production upon overexpression. On the basis of these results, we suggest that CaTHL functions as a key enzyme in the regulation of the main metabolism of C. acetobutylicum through a redox-switch regulatory mechanism. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 1% |
| United States | 1 | 1% |
| Denmark | 1 | 1% |
| Unknown | 94 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 32 | 33% |
| Researcher | 17 | 18% |
| Student > Doctoral Student | 8 | 8% |
| Student > Bachelor | 6 | 6% |
| Student > Master | 6 | 6% |
| Other | 10 | 10% |
| Unknown | 18 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 28 | 29% |
| Agricultural and Biological Sciences | 22 | 23% |
| Chemistry | 8 | 8% |
| Chemical Engineering | 4 | 4% |
| Engineering | 3 | 3% |
| Other | 12 | 12% |
| Unknown | 20 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 45. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2016.
All research outputs
#782,428
of 22,829,083 outputs
Outputs from Nature Communications
#13,139
of 47,014 outputs
Outputs of similar age
#12,340
of 274,417 outputs
Outputs of similar age from Nature Communications
#225
of 755 outputs
Altmetric has tracked 22,829,083 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 47,014 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.7. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 274,417 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 755 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.