Title |
Plasmonic Nanopores for Trapping, Controlling Displacement, and Sequencing of DNA
|
---|---|
Published in |
ACS Nano, October 2015
|
DOI | 10.1021/acsnano.5b04173 |
Pubmed ID | |
Authors |
Maxim Belkin, Shu-Han Chao, Magnus P Jonsson, Cees Dekker, Aleksei Aksimentiev |
Abstract |
With the aim of developing a DNA sequencing methodology, we theoretically examine the feasibility of using nanoplasmonics to control the translocation of a DNA molecule through a solid-state nanopore and to read off sequence information using surface enhanced Raman spectroscopy. Using molecular dynamics simulations, we show that high-intensity optical hot spots produced by a metallic nanostructure can arrest DNA translocation through a solid-state nanopore, thus providing a physical knob for controlling the DNA speed. Switching the plasmonic field on and off can displace the DNA molecule in discrete steps, sequentially exposing neighboring fragments of a DNA molecule to the pore as well as to the plasmonic hot spot. Surface enhanced Raman scattering from the exposed DNA fragments reports on their nucleotide composition, might allow for identification of the nucleotide sequence of a DNA molecule transported through the hot spot. The principles of plasmonic-nanopore sequencing can be extended to detection of DNA modifications and RNA characterization. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 5 | 38% |
United States | 3 | 23% |
Unknown | 5 | 38% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 7 | 54% |
Scientists | 5 | 38% |
Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
Netherlands | 1 | <1% |
Belgium | 1 | <1% |
United Kingdom | 1 | <1% |
Japan | 1 | <1% |
Spain | 1 | <1% |
Unknown | 197 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 57 | 28% |
Researcher | 32 | 16% |
Student > Master | 26 | 13% |
Student > Bachelor | 17 | 8% |
Student > Doctoral Student | 11 | 5% |
Other | 30 | 15% |
Unknown | 32 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 49 | 24% |
Engineering | 40 | 20% |
Chemistry | 38 | 19% |
Materials Science | 13 | 6% |
Biochemistry, Genetics and Molecular Biology | 9 | 4% |
Other | 21 | 10% |
Unknown | 35 | 17% |