Acute ammonia toxicity to the larvae (glochidia) of the tropical Australian freshwater mussel Velesunio spp. Using a modified toxicity test protocol

Linda S. Kleinhenz, Melanie A. Trenfield, Thomas J. Mooney, Christopher L. Humphrey, Rick A. van Dam, Dayanthi Nugegoda, Andrew J. Harford

Ammonia is recognized as a major pollutant worldwide, originating from natural and anthropogenic sources. Studies have reported that freshwater mussels are among the most sensitive taxa to ammonia, but few data are available on ammonia toxicity for the early life stages of freshwater mussels from tropical regions. We report on the modification of a 24-h acute toxicity test protocol for tropical freshwater mussels and application of the test using ammonia. Velesunio spp. from 3 different sites were used to assess the toxicity of ammonia at a targeted pH of 6.0 and a water temperature of 27.5 °C, which were the average annual values for some slightly to moderately acidic, soft water (3-6 mg/L as CaCO3 ) creeks of tropical northern Australia. The valve closure responses of mussel glochidia (larvae) to a sodium chloride solution were used to measure the survival endpoint. Acute toxicity estimates indicate that tropical Velesunio spp. were highly sensitive to ammonia, with 24-h exposures to ammonium sulfate generating median lethal concentration estimates ranging from 6.8 to 14.2 mg/L total ammonia nitrogen, which, when adjusted to pH 7 and 20 °C, were among the highest sensitivities yet reported for any freshwater mussel species, and among the highest in sensitivity for any tropical taxon. These toxicity estimates can contribute to the derivation or refinement of ammonia guideline values for freshwater ecosystems globally. Environ Toxicol Chem 2018;37:2175-2187. © 2018 SETAC.