| Chapter title |
Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
|
|---|---|
| Chapter number | 12 |
| Book title |
Vibrio Cholerae
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8685-9_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8684-2, 978-1-4939-8685-9
|
| Authors |
Aleksandra E. Sikora, Richard Tehan, Kerry McPhail, Sikora, Aleksandra E., Tehan, Richard, McPhail, Kerry |
| Abstract |
The development of antibiotic-resistant bacteria requires increasing research efforts in drug discovery. Vibrio cholerae can be utilized as a model gram-negative enteric pathogen in high- and medium-throughput screening campaigns to identify antimicrobials with different modes of action. In this chapter, we describe methods for the optimal growth of V. cholerae in 384-well plates, preparation of suitable microtiter natural product sample libraries, as well as their screening using measurements of bacterial density and activity of type II secretion-dependent protease as readouts. Concomitant LC-MS/MS profiling and spectral data networking of assay sample libraries facilitate dereplication of putative known and/or nuisance compounds and efficient prioritization of samples containing putative new natural products for further investigation. |
X Demographics
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| Country | Count | As % |
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| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
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| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 25% |
| Student > Bachelor | 1 | 25% |
| Unknown | 2 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Immunology and Microbiology | 1 | 25% |
| Unknown | 2 | 50% |