| Title |
Establishment and characterization of a caudal fin-derived cell line, AOF, from the Oscar, Astronotus ocellatus
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, July 2018
|
| DOI | 10.1007/s10695-018-0542-9 |
| Pubmed ID | |
| Authors |
Raj Kumar, Charan Ravi, Sweta Das, Arathi Dharmaratnam, Valaparambil Saidumohammad Basheer, Thangaraj Raja Swaminathan |
| Abstract |
Astronotus ocellatus, commonly called the oscar, is one of the popular cichlids among aquarium hobby. The present study deals with the development and characterization of a new cell line from caudal fin of A. ocellatus. The cell line was cultured in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28 °C. The optimum temperature and FBS concentration for cell growth were tested with temperature ranges from 20 to 37 °C and FBS concentrations of 5-20% at 28 °C. The Astronotus ocellatus fin cell line has been subcultured 45 times since its development and the modal chromosome number (2n) is 48. The cell line is composed mainly of epithelial cells as confirmed by immunocytological technique using anti-cytokeratin antibodies. The cell line was cryopreserved at different passage levels and the revival efficiency showed 80% survival rate. Partial sequence amplification and sequencing of two genes, mitochondrial 16S ribosomal RNA and cytochrome oxidase I, confirmed the origin of cell line. The cell line did not show Mycoplasma contamination. The cells showed good transfection efficiency when transfected with 2 μg of pAcGFP1-N1 expression vector. The extracellular products of fish bacterial pathogens viz., Aeromonas hydrophila and A. caviae, were cytotoxic to AOF cells but were not susceptible to Cyprinid herpes virus 2. The development of AOF cell line will have significant applications in fish virology and will prove useful to isolate pathogens in the event of sudden viral disease outbreak and for the development of vaccines and diagnostic kits. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 18% |
| Student > Master | 3 | 14% |
| Other | 2 | 9% |
| Student > Doctoral Student | 2 | 9% |
| Student > Ph. D. Student | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 7 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 36% |
| Biochemistry, Genetics and Molecular Biology | 5 | 23% |
| Unspecified | 1 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 August 2018.
All research outputs
#15,542,250
of 23,098,660 outputs
Outputs from Fish Physiology and Biochemistry
#252
of 867 outputs
Outputs of similar age
#209,966
of 330,319 outputs
Outputs of similar age from Fish Physiology and Biochemistry
#9
of 26 outputs
Altmetric has tracked 23,098,660 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 867 research outputs from this source. They receive a mean Attention Score of 1.6. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,319 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.