| Title |
HILIC/ESI-MS determination of gangliosides and other polar lipid classes in renal cell carcinoma and surrounding normal tissues
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, July 2018
|
| DOI | 10.1007/s00216-018-1263-8 |
| Pubmed ID | |
| Authors |
Roman Hájek, Miroslav Lísa, Maria Khalikova, Robert Jirásko, Eva Cífková, Vladimír Študent, David Vrána, Lukáš Opálka, Kateřina Vávrová, Marcel Matzenauer, Bohuslav Melichar, Michal Holčapek |
| Abstract |
Negative-ion hydrophilic liquid chromatography-electrospray ionization mass spectrometry (HILIC/ESI-MS) method has been optimized for the quantitative analysis of ganglioside (GM3) and other polar lipid classes, such as sulfohexosylceramides (SulfoHexCer), sulfodihexosylceramides (SulfoHex2Cer), phosphatidylglycerols (PG), phosphatidylinositols (PI), lysophosphatidylinositols (LPI), and phosphatidylserines (PS). The method is fully validated for the quantitation of the studied lipids in kidney normal and tumor tissues of renal cell carcinoma (RCC) patients based on the lipid class separation and the coelution of lipid class internal standard with the species from the same lipid class. The raw data are semi-automatically processed using our software LipidQuant and statistically evaluated using multivariate data analysis (MDA) methods, which allows the complete differentiation of both groups with 100% specificity and sensitivity. In total, 21 GM3, 28 SulfoHexCer, 26 SulfoHex2Cer, 10 PG, 19 PI, 4 LPI, and 7 PS are determined in the aqueous phase of lipidomic extracts from kidney tumor tissue samples and surrounding normal tissue samples of 20 RCC patients. S-plots allow the identification of most upregulated (PI 40:5, PI 40:4, GM3 34:1, and GM3 42:2) and most downregulated (PI 32:0, PI 34:0, PS 36:4, and LPI 16:0) lipids, which are primarily responsible for the differentiation of tumor and normal groups. Another confirmation of most dysregulated lipids is performed by the calculation of fold changes together with T and p values to highlight their statistical significance. The comparison of HILIC/ESI-MS data and matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) data confirms that lipid dysregulation patterns are similar for both methods. Graphical abstract ᅟ. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Peru | 1 | 20% |
| Mexico | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 60% |
| Members of the public | 1 | 20% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 9 | 20% |
| Student > Ph. D. Student | 8 | 18% |
| Student > Bachelor | 4 | 9% |
| Researcher | 4 | 9% |
| Other | 3 | 7% |
| Other | 8 | 18% |
| Unknown | 9 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 10 | 22% |
| Agricultural and Biological Sciences | 9 | 20% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Neuroscience | 2 | 4% |
| Medicine and Dentistry | 2 | 4% |
| Other | 5 | 11% |
| Unknown | 12 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2018.
All research outputs
#16,053,755
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Outputs from Analytical & Bioanalytical Chemistry
#4,983
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Outputs of similar age
#196,320
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#58
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