| Title |
Isolation and Characterization of Bacteria That Use Furans as the Sole Carbon Source
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, September 2015
|
| DOI | 10.1007/s12010-015-1859-9 |
| Pubmed ID | |
| Authors |
Sarah A. Lee, Lindsey J. Wrona, A. Bruce Cahoon, Jacob Crigler, Mark A. Eiteman, Elliot Altman |
| Abstract |
Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Pseudomonas mendocina (ALS1131), and Pseudomonas putida (ALS1267). In all cases, growth under oxygenated conditions on furfural was accompanied by the transient accumulation of 2-furoic acid (furoate) with no furfuryl alcohol observed. ALS1267 and ALS1279 were also able to metabolize 5-(hydroxymethyl)furfural. The five isolates and their phylogenetic near neighbors were compared for furfural dehydrogenase activity and tolerance to furfural and furoate in defined and complex media. P. putida ALS1267 was the most tolerant to furans and tolerated 17 mM furfural or 195 mM furoate before its growth rate was reduced by 50 % in a defined medium. This strain also had the greatest specific growth rate on furfural (0.6/h at 27-30 °C) and showed the highest specific activity of furfural dehydrogenase (170 mIU/mg) of any furfural-utilizing strain that has been characterized to date. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 7% |
| Canada | 1 | 7% |
| Unknown | 12 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 29% |
| Student > Ph. D. Student | 3 | 21% |
| Student > Bachelor | 2 | 14% |
| Professor | 1 | 7% |
| Lecturer | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 29% |
| Agricultural and Biological Sciences | 2 | 14% |
| Chemistry | 2 | 14% |
| Environmental Science | 1 | 7% |
| Computer Science | 1 | 7% |
| Other | 3 | 21% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2015.
All research outputs
#14,826,358
of 22,829,683 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,513
of 2,508 outputs
Outputs of similar age
#151,439
of 274,379 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#12
of 44 outputs
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So far Altmetric has tracked 2,508 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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