| Title |
CXCR4/CXCL12 signaling impacts enamel progenitor cell proliferation and motility in the dental stem cell niche
|
|---|---|
| Published in |
Cell and Tissue Research, August 2015
|
| DOI | 10.1007/s00441-015-2248-y |
| Pubmed ID | |
| Authors |
Tamaki Yokohama-Tamaki, Keishi Otsu, Hidemitsu Harada, Shunichi Shibata, Nobuko Obara, Kazuharu Irie, Akiyoshi Taniguchi, Takashi Nagasawa, Kazunari Aoki, Steven R. Caliari, Daniel W. Weisgerber, Brendan A. C. Harley |
| Abstract |
Dental stem cells are located at the proximal ends of rodent incisors. These stem cells reside in the dental epithelial stem cell niche, termed the apical bud. We focused on identifying critical features of a chemotactic signal in the niche. Here, we report that CXCR4/CXCL12 signaling impacts enamel progenitor cell proliferation and motility in dental stem cell niche cells. We report cells in the apical bud express CXCR4 mRNA at high levels while expression is restricted in the basal epithelium (BE) and transit-amplifying (TA) cell regions. Furthermore, the CXCL12 ligand is present in mesenchymal cells adjacent to the apical bud. We then performed gain- and loss-of-function analyses to better elucidate the role of CXCR4 and CXCL12. CXCR4-deficient mice contain epithelial cell aggregates, while cell proliferation in mutant incisors was also significantly reduced. We demonstrate in vitro that dental epithelial cells migrate toward sources of CXCL12, whereas knocking down CXCR4 impaired motility and resulted in formation of dense cell colonies. These results suggest that CXCR4 expression may be critical for activation of enamel progenitor cell division and that CXCR4/CXCL12 signaling may control movement of epithelial progenitors from the dental stem cell niche. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Colombia | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 15% |
| Other | 2 | 8% |
| Professor | 2 | 8% |
| Student > Doctoral Student | 2 | 8% |
| Researcher | 2 | 8% |
| Other | 6 | 23% |
| Unknown | 8 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 19% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Agricultural and Biological Sciences | 3 | 12% |
| Social Sciences | 1 | 4% |
| Unspecified | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 11 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 October 2015.
All research outputs
#19,236,357
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#1,706
of 2,279 outputs
Outputs of similar age
#192,567
of 266,182 outputs
Outputs of similar age from Cell and Tissue Research
#16
of 25 outputs
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