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PKCε as a novel promoter of skeletal muscle differentiation and regeneration

Overview of attention for article published in Experimental Cell Research, September 2015
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  • Above-average Attention Score compared to outputs of the same age (54th percentile)
  • Good Attention Score compared to outputs of the same age and source (65th percentile)

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Title
PKCε as a novel promoter of skeletal muscle differentiation and regeneration
Published in
Experimental Cell Research, September 2015
DOI 10.1016/j.yexcr.2015.09.017
Pubmed ID
Authors

D. Di Marcantonio, D. Galli, C. Carubbi, G. Gobbi, V. Queirolo, S. Martini, S. Merighi, M. Vaccarezza, N. Maffulli, S.M. Sykes, M. Vitale, P. Mirandola

Abstract

Satellite cells are muscle resident stem cells and are responsible for muscle regeneration. In this study we investigate the involvement of PKCε during muscle stem cell differentiation in vitro and in vivo. Here, we describe the identification of a previously unrecognized role for the PKCε - HMGA1 signaling axis in myoblast differentiation and regeneration processes. PKCε expression was modulated in the C2C12 cell line and primary murine satellite cells in vitro, as well as in an in vivo model of muscle regeneration. Immunohistochemistry and immunofluorescence, RT-PCR and shRNA silencing techniques were used to determine the role of PKCε and HMGA1 in myogenic differentiation. PKCε expression increases and subsequently re-localizes to the nucleus during skeletal muscle cell differentiation. In the nucleus, PKCε blocks Hmga1 expression to promote Myogenin and Mrf4 accumulation and myoblast formation. Following in vivo muscle injury, PKCε accumulates in regenerating, centrally-nucleated myofibers. Pharmacological inhibition of PKCε impairs the expression of two crucial markers of muscle differentiation, namely MyoD and Myogenin, during injury induced muscle regeneration. This work identifies the PKCε - HMGA1 signaling axis as a positive regulator of skeletal muscle differentiation.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 15%
Student > Bachelor 4 15%
Researcher 3 11%
Student > Ph. D. Student 3 11%
Other 2 7%
Other 7 26%
Unknown 4 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 44%
Biochemistry, Genetics and Molecular Biology 6 22%
Medicine and Dentistry 2 7%
Sports and Recreations 1 4%
Materials Science 1 4%
Other 0 0%
Unknown 5 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2016.
All research outputs
#14,388,865
of 25,374,917 outputs
Outputs from Experimental Cell Research
#3,656
of 5,318 outputs
Outputs of similar age
#129,191
of 286,342 outputs
Outputs of similar age from Experimental Cell Research
#20
of 58 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,318 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 286,342 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.