| Title |
Serum levels of inflammatory cytokines in Rift Valley fever patients are indicative of severe disease
|
|---|---|
| Published in |
Virology Journal, October 2015
|
| DOI | 10.1186/s12985-015-0392-3 |
| Pubmed ID | |
| Authors |
Petrus Jansen van Vuren, Sharon Shalekoff, Antoinette A. Grobbelaar, Brett N. Archer, Juno Thomas, Caroline T. Tiemessen, Janusz T. Paweska |
| Abstract |
Rift Valley fever (RVF) is a mosquito-borne viral zoonosis affecting domestic and wild ruminants, camels and humans. Outbreaks of RVF are characterized by a sudden onset of abortions and high mortality amongst domestic ruminants. Humans develop disease ranging from a mild flu-like illness to more severe complications including hemorrhagic syndrome, ocular and neurological lesions and death. During the RVF outbreak in South Africa in 2010/11, a total of 278 human cases were laboratory confirmed, including 25 deaths. The role of the host inflammatory response to RVF pathogenesis is not completely understood. Virus load in serum from human fatal and non-fatal cases was determined by standard tissue culture infective dose 50 (TCID50) titration on Vero cells. Patient serum concentration of chemokines and cytokines involved in inflammatory responses (IL-8, RANTES, CXCL9, MCP-1, IP-10, IL-1β, IL-6, IL-10, TNF and IL-12p70) was determined using cytometric bead assays and flow cytometry. Fatal cases had a 1-log10 higher TCID50/ml serum concentration of RVF virus (RVFV) than survivors (p < 0.05). There were no significant sequence differences between isolates recovered from fatal and non-fatal cases. Chemokines and pro- and anti-inflammatory cytokines were detected at significantly increased (IL-8, CXCL9, MCP-1, IP-10, IL-10) or decreased (RANTES) levels when comparing fatal cases to infected survivors and uninfected controls, or when comparing combined infected patients to uninfected controls. The results suggest that regulation of the host inflammatory responses plays an important role in the outcome of RVFV infection in humans. Dysregulation of the inflammatory response contributes to a fatal outcome. The cytokines and chemokines identified in this study that correlate with fatal outcomes warrant further investigation as markers for disease severity. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 88% |
| Science communicators (journalists, bloggers, editors) | 1 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 65 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 18% |
| Student > Master | 8 | 12% |
| Student > Ph. D. Student | 6 | 9% |
| Student > Bachelor | 4 | 6% |
| Student > Postgraduate | 4 | 6% |
| Other | 11 | 17% |
| Unknown | 20 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 17% |
| Immunology and Microbiology | 8 | 12% |
| Veterinary Science and Veterinary Medicine | 6 | 9% |
| Medicine and Dentistry | 6 | 9% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Other | 9 | 14% |
| Unknown | 21 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2021.
All research outputs
#6,717,492
of 22,829,683 outputs
Outputs from Virology Journal
#727
of 3,043 outputs
Outputs of similar age
#81,974
of 277,991 outputs
Outputs of similar age from Virology Journal
#11
of 57 outputs
Altmetric has tracked 22,829,683 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 3,043 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.8. This one has done well, scoring higher than 75% of its peers.
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We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.