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Histone Variants

Overview of attention for book
Cover of 'Histone Variants'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Methods for Preparing Nucleosomes Containing Histone Variants
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    Chapter 2 Characterization of Posttranslational Modifications on Histone Variants
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    Chapter 3 Purification of Histone Variant-Interacting Chaperone Complexes
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    Chapter 4 Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics
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    Chapter 5 Histone Native Chromatin Immunoprecipitation
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    Chapter 6 How to Tackle Challenging ChIP-Seq, with Long-Range Cross-Linking, Using ATRX as an Example
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    Chapter 7 time-ChIP: A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance
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    Chapter 8 MINCE-Seq: Mapping In Vivo Nascent Chromatin with EdU and Sequencing
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    Chapter 9 RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes
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    Chapter 10 Bioinformatic Analysis of Nucleosome and Histone Variant Positioning
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    Chapter 11 Imaging Newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System
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    Chapter 12 Real-Time De Novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin-Inducible Degradation System
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    Chapter 13 Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin
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    Chapter 14 CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A
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    Chapter 15 Studying the Evolution of Histone Variants Using Phylogeny
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    Chapter 16 Characterization of Post-Meiotic Male Germ Cell Genome Organizational States
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    Chapter 17 An Animal Model for Genetic Analysis of Multi-Gene Families: Cloning and Transgenesis of Large Tandemly Repeated Histone Gene Clusters
  19. Altmetric Badge
    Chapter 18 Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH)
  20. Altmetric Badge
    Chapter 19 Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations
Attention for Chapter 14: CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A
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Chapter title
CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A
Chapter number 14
Book title
Histone Variants
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8663-7_14
Pubmed ID
Book ISBNs
978-1-4939-8662-0, 978-1-4939-8663-7
Authors

Justin W. C. Leung, Lara E. Emery, Kyle M. Miller

Abstract

Histone H2A variants play important roles in maintaining the integrity of the genome. For example, the histone variant H2AX is phosphorylated on Ser139 (called γH2AX) at DNA double-strand breaks (DSB) and serves as a signal for the initiation of downstream DNA damage response (DDR) factor recruitment and DNA repair activities within damaged chromatin. For decades, genetic studies in human cells involving DNA damage signaling and repair factors have relied mostly on either knockdown by RNA interference (i.e., shRNA and siRNA) or the use of mouse embryonic fibroblasts derived from knockout (KO) mice. Recent advances in gene editing using ZNF nuclease, TALEN, and CRISPR/Cas9 have allowed the generation of human KO cell lines, allowing genetic models for studying the DDR, including histone H2A variants in human cells. Here, we describe a detailed protocol for generating and verifying KO of H2AX and macroH2A histone H2A variants using CRISPR/Cas9 gene editing in human cancer cell lines. This protocol allows the use and development of genetic systems in human cells to study histone variants and their functions, including within the DDR.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 46%
Student > Master 2 15%
Student > Doctoral Student 2 15%
Professor > Associate Professor 1 8%
Student > Bachelor 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 69%
Agricultural and Biological Sciences 3 23%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2018.
All research outputs
#15,542,250
of 23,098,660 outputs
Outputs from Methods in molecular biology
#5,412
of 13,208 outputs
Outputs of similar age
#210,055
of 331,034 outputs
Outputs of similar age from Methods in molecular biology
#82
of 193 outputs
Altmetric has tracked 23,098,660 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,034 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 193 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.