| Title |
OsRACK1A, encodes a circadian clock-regulated WD40 protein, negatively affect salt tolerance in rice
|
|---|---|
| Published in |
Rice, August 2018
|
| DOI | 10.1186/s12284-018-0232-3 |
| Pubmed ID | |
| Authors |
Dongping Zhang, Yuzhu Wang, Jinyu Shen, Jianfeng Yin, Dahong Li, Yan Gao, Weifeng Xu, Jiansheng Liang |
| Abstract |
The receptor for activated C kinase 1 (RACK1) is a WD40 type protein that is involved in multiple signaling pathways and is conserved from prokaryotes to eukaryotes. Here we report that rice RACK1A (OsRACK1A) is regulated by circadian clocks and plays an important role in the salt stress response. OsRACK1A was found to follow a rhythmic expression profile under circadian conditions at both the transcription and the translation levels, although the expression was arrhythmic under salt stress. Analysis of plant survival rates, fresh weight, proline content, malondialdehyde, and chlorophyll showed that suppression of OsRACK1A enhanced tolerance to salt stress. The ion concentration in both roots and leaves revealed that OsRACK1A-suppressed transgenic rice could maintain low Na+ and high K+ concentrations. Furthermore, OsRACK1A-suppressed transgenic rice accumulated significantly more abscisic acid (ABA) and more transcripts of ABA- and stress-inducible genes compared with the wild-type plants. Real-time quantitative polymerase chain reaction analysis revealed that many stress-related genes, including APETALA 2/Ethylene Responsive Factor (AP2/ERF) transcription factors, were upregulated in the OsRACK1A-suppressed transgenic rice line. We identified putative interactors of OsRACK1A, and found that OsRACK1A interacted with many salt stress-responsive proteins directly. These results suggest that OsRACK1A is regulated by circadian rhythm, and involved in the regulation of salt stress responses. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 31% |
| Researcher | 5 | 19% |
| Student > Master | 3 | 12% |
| Student > Bachelor | 1 | 4% |
| Other | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 7 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 42% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Physics and Astronomy | 1 | 4% |
| Energy | 1 | 4% |
| Unknown | 9 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2018.
All research outputs
#18,645,475
of 23,098,660 outputs
Outputs from Rice
#254
of 391 outputs
Outputs of similar age
#254,592
of 331,122 outputs
Outputs of similar age from Rice
#4
of 10 outputs
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