| Title |
FAT1 cadherin acts upstream of Hippo signalling through TAZ to regulate neuronal differentiation
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, June 2015
|
| DOI | 10.1007/s00018-015-1955-6 |
| Pubmed ID | |
| Authors |
Abdulrzag F. Ahmed, Charles E. de Bock, Lisa F. Lincz, Jay Pundavela, Ihssane Zouikr, Estelle Sontag, Hubert Hondermarck, Rick F. Thorne |
| Abstract |
The Hippo pathway is emerging as a critical nexus that balances self-renewal of progenitors against differentiation; however, upstream elements in vertebrate Hippo signalling are poorly understood. High expression of Fat1 cadherin within the developing neuroepithelium and the manifestation of severe neurological phenotypes in Fat1-knockout mice suggest roles in neurogenesis. Using the SH-SY5Y model of neuronal differentiation and employing gene silencing techniques, we show that FAT1 acts to control neurite outgrowth, also driving cells towards terminal differentiation via inhibitory effects on proliferation. FAT1 actions were shown to be mediated through Hippo signalling where it activated core Hippo kinase components and antagonised functions of the Hippo effector TAZ. Suppression of FAT1 promoted the nucleocytoplasmic shuttling of TAZ leading to enhanced transcription of the Hippo target gene CTGF together with accompanying increases in nuclear levels of Smad3. Silencing of TAZ reversed the effects of FAT1 depletion thus connecting inactivation of TAZ-TGFbeta signalling with Hippo signalling mediated through FAT1. These findings establish FAT1 as a new upstream Hippo element regulating early stages of differentiation in neuronal cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 5% |
| Poland | 1 | 2% |
| Unknown | 53 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 26% |
| Researcher | 11 | 19% |
| Student > Master | 9 | 16% |
| Professor > Associate Professor | 3 | 5% |
| Student > Bachelor | 2 | 4% |
| Other | 4 | 7% |
| Unknown | 13 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 30% |
| Biochemistry, Genetics and Molecular Biology | 11 | 19% |
| Neuroscience | 4 | 7% |
| Medicine and Dentistry | 4 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 17 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2015.
All research outputs
#7,845,540
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#1,655
of 4,151 outputs
Outputs of similar age
#90,815
of 265,362 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#26
of 65 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,151 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,362 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.