Title |
HDXFinder: Automated Analysis and Data Reporting of Deuterium/Hydrogen Exchange Mass Spectrometry
|
---|---|
Published in |
Journal of the American Society for Mass Spectrometry, November 2011
|
DOI | 10.1007/s13361-011-0234-5 |
Pubmed ID | |
Authors |
Danny E. Miller, Charulata B. Prasannan, Maria T. Villar, Aron W. Fenton, Antonio Artigues |
Abstract |
Hydrogen/deuterium exchange in combination with mass spectrometry (H/D MS) is a sensitive technique for detection of changes in protein conformation and dynamics. However, wide application of H/D MS has been hindered, in part, by the lack of computational tools necessary for efficient analysis of the large data sets associated with this technique. We report a novel web-based application for automatic analysis of H/D MS experimental data. This application relies on the high resolution of mass spectrometers to extract all isotopic envelopes before correlating these envelopes with individual peptides. Although a fully automatic analysis is possible, a variety of graphical tools are included to aid in the verification of correlations and rankings of the isotopic peptide envelopes. As a demonstration, the rate constants for H/D exchange of peptides from rabbit muscle pyruvate kinase are mapped onto the structure of this protein. |
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Geographical breakdown
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India | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 13% |
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Student > Bachelor | 2 | 8% |
Student > Master | 2 | 8% |
Other | 4 | 17% |
Unknown | 1 | 4% |
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Other | 1 | 4% |
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