| Title |
Expression of ADAMTS-4 by chondrocytes in the surface zone of human osteoarthritic cartilage is regulated by epigenetic DNA de-methylation
|
|---|---|
| Published in |
Rheumatology International, October 2008
|
| DOI | 10.1007/s00296-008-0744-z |
| Pubmed ID | |
| Authors |
Kelvin S. C. Cheung, Ko Hashimoto, Norikazu Yamada, Helmtrud I. Roach |
| Abstract |
The two major aggrecanases involved in osteoarthritis (OA) are ADAMTS-4 and ADAMTS-5. Knock-out studies suggested that ADAMTS-5, but not ADAMTS-4, is the major aggrecanase in murine OA. However, studies of human articular cartilage suggest that ADAMTS-4 also contributes to aggrecan degradation in human OA. This study investigated ADAMTS-4 in human OA. While ADAMTS-4 was virtually absent in control cartilage, numerous ADAMTS-4 immuno-positive chondrocytes were present in OA cartilage and their numbers increased with disease severity. RT-PCR confirmed expression, especially in the surface zone. DNA methylation was lost at specific CpG sites in the ADAMTS-4 promoter in OA chondrocytes, suggesting that the increased gene expression was more than a simple up-regulation, but involved loss of DNA methylation at specific CpG sites, resulting in a heritable and permanent expression of ADAMTS-4 in OA chondrocytes. These results suggest that ADAMTS-4 is epigenetically regulated and plays a role in aggrecan degradation in human OA. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| Australia | 1 | 1% |
| Italy | 1 | 1% |
| Mexico | 1 | 1% |
| Spain | 1 | 1% |
| Unknown | 61 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 24% |
| Researcher | 13 | 19% |
| Student > Master | 7 | 10% |
| Student > Doctoral Student | 7 | 10% |
| Student > Postgraduate | 4 | 6% |
| Other | 14 | 21% |
| Unknown | 6 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 34% |
| Biochemistry, Genetics and Molecular Biology | 13 | 19% |
| Medicine and Dentistry | 12 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
| Immunology and Microbiology | 1 | 1% |
| Other | 7 | 10% |
| Unknown | 10 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2015.
All research outputs
#15,348,897
of 22,830,751 outputs
Outputs from Rheumatology International
#1,539
of 2,180 outputs
Outputs of similar age
#77,284
of 91,210 outputs
Outputs of similar age from Rheumatology International
#14
of 15 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,180 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.