| Title |
Transplantation of rat embryonic stem cell-derived retinal cells restores visual function in the Royal College of Surgeons rats
|
|---|---|
| Published in |
Documenta Ophthalmologica, August 2018
|
| DOI | 10.1007/s10633-018-9648-8 |
| Pubmed ID | |
| Authors |
Hongxi Wu, Jia Li, Xinbang Mao, Guodong Li, Lin Xie, Zhipeng You |
| Abstract |
To evaluate the feasibility of transplantation of embryonic stem cell (ESC)-derived retinal cells in the treatment of retinal degeneration. Rat ESCs were isolated and induced into retinal progenitor cells (RPCs) in vitro, which were subsequently induced into retinal pigment epithelium cells (RPEs) and photoreceptors (PRCs). All cells were identified by Western blot detection of their specific markers. RPEs and PRCs were, respectively, injected into the retina of Royal College of Surgeons (RCSs) rats. Control group was injected with PBS. Post-transplantation visual function was determined by electroretinography (ERG). The histology of the whole eye was compared by H&E staining. RPEs and PRCs were successfully derived from rat ESCs through the two-step differentiation as indicated by the presence of ESC- (Oct-3/4, Nanog, TRA-1-60 and TRA-1-81), RPC- (Rx, Mitf, Pax6 and Chx10), RPE- (RPE65 and keratin) and PRC-specific markers (blue opsin, red/green opsin, recoverin and rhodopsin) in Western blot. The amplitude of ERG a- and b-wave in RPE- and PRC-transplanted groups at week 2 and 10 after transplantation was markedly higher compared with PBS controls. Retinal injury and vascular stress response was not detected in any of the RCS rats after transplantation. The developed stepwise protocol can derive retinal cells from ESCs. Transplantation of these retinal cells can restore visual function of RCS rats. Our study provides evidence for potential clinical application of ESC-based cell therapy for retinal degeneration. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 15% |
| Student > Doctoral Student | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Student > Master | 1 | 8% |
| Researcher | 1 | 8% |
| Other | 2 | 15% |
| Unknown | 5 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 3 | 23% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Medicine and Dentistry | 2 | 15% |
| Unknown | 6 | 46% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2018.
All research outputs
#14,887,436
of 23,099,576 outputs
Outputs from Documenta Ophthalmologica
#250
of 462 outputs
Outputs of similar age
#196,347
of 331,034 outputs
Outputs of similar age from Documenta Ophthalmologica
#5
of 9 outputs
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