| Title |
A novel in vitro model of sarcopenia using BubR1 hypomorphic C2C12 myoblasts
|
|---|---|
| Published in |
Methods in Cell Science, October 2015
|
| DOI | 10.1007/s10616-015-9920-7 |
| Pubmed ID | |
| Authors |
Takateru Nozaki, Shiori Nikai, Ryo Okabe, Kiyoko Nagahama, Nozomu Eto |
| Abstract |
Sarcopenia is the age-related loss of skeletal muscle mass and function with adverse outcomes that include physical disability, poor quality of life, and death. The detailed molecular mechanisms remain unknown. An in vitro muscle atrophy model is needed to enable mechanistic studies. To create such a model, we employed BubR1 insufficiency which causes premature ageing in mice. Using C2C12 cells, a recognized in vitro model of the skeletal muscle cell, we obtained the BubR1 hypomorphic C2C12 (C2C12BKD) cells by using shRNA. The resulting C2C12BKD cells displayed several characteristics of the sarcopenic muscle cell. In C2C12BKD cells, formation of myotubes, assessed by analysis of fusion index, was markedly reduced as was the expression of myogenin and MyoD, two marker genes for myogenesis. Moreover, the cells showed increased expression of the muscle-specific ubiquitin ligases Atrogin-1 and MuRF-1, indicating increased protein degradation through the ubiquitin-proteasome dependent proteolytic pathway. These results suggest that C2C12BKD cells are potentially useful as a novel in vitro model of sarcopenia. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Norway | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 57 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 23% |
| Student > Master | 10 | 18% |
| Student > Bachelor | 5 | 9% |
| Researcher | 3 | 5% |
| Student > Doctoral Student | 2 | 4% |
| Other | 5 | 9% |
| Unknown | 19 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 14% |
| Medicine and Dentistry | 6 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
| Sports and Recreations | 3 | 5% |
| Other | 6 | 11% |
| Unknown | 19 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2023.
All research outputs
#7,356,343
of 25,374,647 outputs
Outputs from Methods in Cell Science
#322
of 1,026 outputs
Outputs of similar age
#84,191
of 291,306 outputs
Outputs of similar age from Methods in Cell Science
#3
of 10 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 1,026 research outputs from this source. They receive a mean Attention Score of 4.7. This one has gotten more attention than average, scoring higher than 67% of its peers.
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We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 7 of them.