| Title |
Functionalization of stable fluorescent nanodiamonds towards reliable detection of biomarkers for Alzheimer’s disease
|
|---|---|
| Published in |
Journal of Nanobiotechnology, August 2018
|
| DOI | 10.1186/s12951-018-0385-7 |
| Pubmed ID | |
| Authors |
Francisco Morales-Zavala, Nathalie Casanova-Morales, Raúl B. Gonzalez, América Chandía-Cristi, Lisbell D. Estrada, Ignacio Alvizú, Victor Waselowski, Fanny Guzman, Simón Guerrero, Marisol Oyarzún-Olave, Cristian Rebolledo, Enrique Rodriguez, Julien Armijo, Heman Bhuyan, Mario Favre, Alejandra R. Alvarez, Marcelo J. Kogan, Jerónimo R. Maze |
| Abstract |
Stable and non-toxic fluorescent markers are gaining attention in molecular diagnostics as powerful tools for enabling long and reliable biological studies. Such markers should not only have a long half-life under several assay conditions showing no photo bleaching or blinking but also, they must allow for their conjugation or functionalization as a crucial step for numerous applications such as cellular tracking, biomarker detection and drug delivery. We report the functionalization of stable fluorescent markers based on nanodiamonds (NDs) with a bifunctional peptide. This peptide is made of a cell penetrating peptide and a six amino acids long β-sheet breaker peptide that is able to recognize amyloid β (Aβ) aggregates, a biomarker for the Alzheimer disease. Our results indicate that functionalized NDs (fNDs) are not cytotoxic and can be internalized by the cells. The fNDs allow ultrasensitive detection (at picomolar concentrations of NDs) of in vitro amyloid fibrils and amyloid aggregates in AD mice brains. The fluorescence of functionalized NDs is more stable than that of fluorescent markers commonly used to stain Aβ aggregates such as Thioflavin T. These results pave the way for performing ultrasensitive and reliable detection of Aβ aggregates involved in the pathogenesis of the Alzheimer disease. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 56 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 16% |
| Student > Bachelor | 9 | 16% |
| Researcher | 8 | 14% |
| Student > Ph. D. Student | 7 | 13% |
| Librarian | 3 | 5% |
| Other | 7 | 13% |
| Unknown | 13 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 14% |
| Physics and Astronomy | 8 | 14% |
| Engineering | 5 | 9% |
| Agricultural and Biological Sciences | 4 | 7% |
| Chemistry | 4 | 7% |
| Other | 12 | 21% |
| Unknown | 15 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2021.
All research outputs
#2,220,087
of 23,099,576 outputs
Outputs from Journal of Nanobiotechnology
#62
of 1,451 outputs
Outputs of similar age
#47,821
of 331,118 outputs
Outputs of similar age from Journal of Nanobiotechnology
#3
of 20 outputs
Altmetric has tracked 23,099,576 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,451 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 95% of its peers.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.