| Title |
Evidence for complete nitrification in enrichment culture of tidal sediments and diversity analysis of clade a comammox Nitrospira in natural environments
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, August 2018
|
| DOI | 10.1007/s00253-018-9274-0 |
| Pubmed ID | |
| Authors |
Chendi Yu, Lijun Hou, Yanling Zheng, Min Liu, Guoyu Yin, Juan Gao, Cheng Liu, Yongkai Chang, Ping Han |
| Abstract |
Complete ammonia oxidizers (comammox), as novel microbial communities, are predicted to play an important role in the nitrogen cycle. Here we reported the presence of complete nitrification in tidal sediments and examined the diversity and abundance of comammox in natural ecosystems. Metagenome and metatranscriptome of the enrichment culture from tidal sediments harbored the genes of comammox. Near-complete comammox AmoA/B/C- and Hao-like sequences showed close relationships to the known comammox (with sequence identity from 79 to 99%) rather than classical betaproteobacterial ammonia-oxidizing bacteria (β-AOB) (57 to 66%) and ammonia-oxidizing archaea (AOA) (24 to 38%). To analyze the diversity of comammox in natural environments, a new primer set targeting clade A comammox Nitrospira (COM-A) amoA genes was designed based on sequences obtained in this study and sequences from published database. In silico evaluation of the primers showed the high coverage of 89 and 100% in the COM-A amoA database. Application of the primers in six different ecosystems proved their strong availability. Community composition of COM-A suggested a relatively higher diversity than β-AOB in similar environments. Quantification results showed that COM-A amoA genes accounted for about 0.4-5.6% in total amoA genes. These results provide novel insight into our perception of the enigmatic comammox and have significant implications for profound understanding of complex nitrification process. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Netherlands | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 62 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 23% |
| Student > Master | 10 | 16% |
| Student > Bachelor | 7 | 11% |
| Researcher | 5 | 8% |
| Professor | 3 | 5% |
| Other | 4 | 6% |
| Unknown | 19 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 14 | 23% |
| Agricultural and Biological Sciences | 10 | 16% |
| Biochemistry, Genetics and Molecular Biology | 6 | 10% |
| Immunology and Microbiology | 4 | 6% |
| Engineering | 2 | 3% |
| Other | 3 | 5% |
| Unknown | 23 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2018.
All research outputs
#4,242,425
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#999
of 8,034 outputs
Outputs of similar age
#77,838
of 334,993 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#17
of 137 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 87% of its peers.
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We're also able to compare this research output to 137 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.