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Mendeley readers
Attention Score in Context
| Title |
Imaging the immunological synapse between dendritic cells and T cells
|
|---|---|
| Published in |
Immunotechnology, May 2015
|
| DOI | 10.1016/j.jim.2015.04.029 |
| Pubmed ID | |
| Authors |
Kate A. Markey, Kate H. Gartlan, Rachel D. Kuns, Kelli P.A. MacDonald, Geoffrey R. Hill |
| Abstract |
Immunological synapse formation between antigen-specific T cells and antigen presenting cells (APC) involves reorganization of the cellular cytoskeleton (polymerization of filamentous actin) and recruitment of adhesion molecules (e.g. LFA-1, ICAM-1). This engagement is critical for the generation of specific immune responses. Until recently, quantitative, high-throughput measurements of these interactions have not been possible. Instead, previous assessment was reliant on qualitative microscopy of live cells, where typically the APC is adhered to a surface and the suspended T cell is required to migrate to facilitate synapse formation. While this methodology can demonstrate the capacity for synapse formation, it cannot accommodate quantification of large numbers of interacting cell pairs, nor does it allow for statistically robust comparison between test conditions. We have developed a method for assessing immunological synapse formation between purified ex vivo dendritic cells (DC) and responder antigen-specific CD4(+) T cells using imaging flow cytometry, allowing us to quantify LFA-1 and f-actin rearrangement at the interface between DC/T cell pairs. This novel application of imaging flow cytometry represents a major advance in dendritic cell function and immunological synapse research as it facilitates quantitative, high throughput analysis of the interaction between live, ex-vivo DC and T cells. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 106 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 27 | 25% |
| Student > Ph. D. Student | 24 | 22% |
| Student > Master | 16 | 15% |
| Student > Bachelor | 12 | 11% |
| Student > Doctoral Student | 4 | 4% |
| Other | 15 | 14% |
| Unknown | 10 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 31 | 29% |
| Immunology and Microbiology | 31 | 29% |
| Biochemistry, Genetics and Molecular Biology | 16 | 15% |
| Medicine and Dentistry | 5 | 5% |
| Chemistry | 4 | 4% |
| Other | 6 | 6% |
| Unknown | 15 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2023.
All research outputs
#4,760,313
of 25,374,647 outputs
Outputs from Immunotechnology
#800
of 4,810 outputs
Outputs of similar age
#55,665
of 278,746 outputs
Outputs of similar age from Immunotechnology
#8
of 45 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,810 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,746 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.