| Title |
Sirtuin 6 regulates glucose-stimulated insulin secretion in mouse pancreatic beta cells
|
|---|---|
| Published in |
Diabetologia, October 2015
|
| DOI | 10.1007/s00125-015-3778-2 |
| Pubmed ID | |
| Authors |
Xiwen Xiong, Gaihong Wang, Rongya Tao, Pengfei Wu, Tatsuyoshi Kono, Kevin Li, Wen-Xing Ding, Xin Tong, Sarah A. Tersey, Robert A. Harris, Raghavendra G. Mirmira, Carmella Evans-Molina, X. Charlie Dong |
| Abstract |
Sirtuin 6 (SIRT6) has been implicated in ageing, DNA repair and metabolism; however, its function in pancreatic beta cells is unclear. The aim of this study is to elucidate the role of SIRT6 in pancreatic beta cells. To investigate the function of SIRT6 in pancreatic beta cells, we performed Sirt6 gene knockdown in MIN6 cells and generated pancreatic- and beta cell-specific Sirt6 knockout mice. Islet morphology and glucose-stimulated insulin secretion (GSIS) were analysed. Glycolysis and oxygen consumption rates in SIRT6-deficient beta cells were measured. Cytosolic calcium was monitored using the Fura-2-AM fluorescent probe (Invitrogen, Grand Island, NY, USA). Mitochondria were analysed by immunoblots and electron microscopy. Sirt6 knockdown in MIN6 beta cells led to a significant decrease in GSIS. Pancreatic beta cell Sirt6 knockout mice showed a ~50% decrease in GSIS. The knockout mouse islets had lower ATP levels compared with the wild-type controls. Mitochondrial oxygen consumption rates were significantly decreased in the SIRT6-deficient beta cells. Cytosolic calcium dynamics in response to glucose or potassium chloride were attenuated in the Sirt6 knockout islets. Numbers of damaged mitochondria were increased and mitochondrial complex levels were decreased in the SIRT6-deficient islets. These data suggest that SIRT6 is important for GSIS from pancreatic beta cells and activation of SIRT6 may be useful to improve insulin secretion in diabetes. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 25% |
| Canada | 1 | 13% |
| United Kingdom | 1 | 13% |
| Unknown | 4 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 75% |
| Practitioners (doctors, other healthcare professionals) | 1 | 13% |
| Science communicators (journalists, bloggers, editors) | 1 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 58 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 22% |
| Student > Ph. D. Student | 11 | 19% |
| Researcher | 6 | 10% |
| Professor > Associate Professor | 4 | 7% |
| Professor | 3 | 5% |
| Other | 8 | 14% |
| Unknown | 13 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 24% |
| Biochemistry, Genetics and Molecular Biology | 13 | 22% |
| Medicine and Dentistry | 7 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Unspecified | 2 | 3% |
| Other | 6 | 10% |
| Unknown | 14 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2023.
All research outputs
#4,430,262
of 24,940,046 outputs
Outputs from Diabetologia
#1,911
of 5,319 outputs
Outputs of similar age
#54,899
of 285,040 outputs
Outputs of similar age from Diabetologia
#24
of 77 outputs
Altmetric has tracked 24,940,046 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,319 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 24.5. This one has gotten more attention than average, scoring higher than 63% of its peers.
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We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.