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Potential role of vitexin in alleviating heat stress-induced cytotoxicity: Regulatory effect of Hsp90 on ER stress-mediated autophagy

Overview of attention for article published in Life Sciences, October 2015
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Title
Potential role of vitexin in alleviating heat stress-induced cytotoxicity: Regulatory effect of Hsp90 on ER stress-mediated autophagy
Published in
Life Sciences, October 2015
DOI 10.1016/j.lfs.2015.10.012
Pubmed ID
Authors

Monika Bhardwaj, Souren Paul, Rekha Jakhar, Sun Chul Kang

Abstract

Cells possess multiple methods for counteracting the deleterious consequences of stress induced by physical and chemical stimuli. Heat stress causes variations in the cellular environment, leading to cellular morbidity or mortality. Natural compounds that contain phenolic antioxidants, offer various therapeutic and biological activities. Vitexin, a natural flavonoid, has been reported to treat various pathologies due to its multifaceted effects. Herein, we investigated the therapeutic efficacy of vitexin and its underlying mechanism against heat stress in human lung epithelial cells. Effect of vitexin on the expression of molecular chaperones, antioxidant enzymes, mitogen activated protein kinases (MAPKs), endoplasmic reticulum (ER)-stress and autophagy were measured by immunoblotting. qRT-PCR and EMSA was performed for Hsp90 expression and HSF-1 binding affinity. Cell viability was assessed by MTT and LDH assay. Detection of autophagy was confirmed by acridine orange staining. Role of Hsp90 inhibition on signaling pathways was elucidated by using specific chemical inhibitor, radicicol. Whereas hyperthermia reduced cell viability, result of MTT and LDH assay showed that vitexin pre-treatment enhanced cell viability after heat stress. EMSA analysis shows DNA binding affinity of HSF-1 during heat stress. Vitexin upregulated Hsp90 expression, subsequently activating ER-stress induced autophagy. Modulation of MAPKs expression and fluorescence image analysis showed vacuole accumulation, indicating autophagic flux in cells. Hsp90 inhibition reversed the effect of vitexin and activates the apoptosis pathway. Our data suggest that vitexin can protect against hyperthermic cellular injury by induction of Hsp90 expression, antioxidant activity and MAPKs via ER stress-induced autophagy.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 19%
Student > Master 3 11%
Lecturer 2 7%
Other 2 7%
Student > Bachelor 2 7%
Other 5 19%
Unknown 8 30%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 4 15%
Chemistry 3 11%
Biochemistry, Genetics and Molecular Biology 2 7%
Mathematics 2 7%
Agricultural and Biological Sciences 2 7%
Other 2 7%
Unknown 12 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2015.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Life Sciences
#6,475
of 7,632 outputs
Outputs of similar age
#251,801
of 294,143 outputs
Outputs of similar age from Life Sciences
#27
of 46 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,632 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.