| Title |
Detecting senescent fate in mesenchymal stem cells: a combined cytofluorimetric and ultrastructural approach
|
|---|---|
| Published in |
Biogerontology, August 2018
|
| DOI | 10.1007/s10522-018-9766-4 |
| Pubmed ID | |
| Authors |
Manuela Dicarlo, Gabriella Teti, Iolanda Iezzi, Giorgia Cerqueni, Sandra Manzotti, Mirella Falconi, Monica Mattioli-Belmonte |
| Abstract |
Senescence can impair the therapeutic potential of stem cells. In this study, senescence-associated morphofunctional changes in periosteum-derived progenitor cells (PDPCs) from old and young individuals were investigated by combining cytofluorimetry, immunohistochemistry, and transmission electron microscopy. Cell cycle analysis demonstrated a large number of G0/G1 phase cells in PDPCs from old subjects and a progressive accumulation of G0/G1 cells during passaging in cultures from young subjects. Cytofluorimetry documented significant changes in light scattering parameters and closely correlated with the ultrastructural features, especially changes in mitochondrial shape and autophagy, which are consistent with the mitochondrial-lysosomal axis theory of ageing. The combined morphological, biofunctional, and ultrastructural approach enhanced the flow cytometric study of PDPC ageing. We speculate that impaired autophagy, documented in replicative senescent and old PDPCs, reflect a switch from quiescence to senescence. Its demonstration in a tissue with limited turnover-like the cambium layer of the periosteum, where reversible quiescence is the normal stem cell state throughout life-adds a new piece to the regenerative medicine jigsaw in an ageing society. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 36% |
| Student > Ph. D. Student | 2 | 18% |
| Student > Doctoral Student | 1 | 9% |
| Lecturer | 1 | 9% |
| Student > Master | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 36% |
| Medicine and Dentistry | 2 | 18% |
| Computer Science | 1 | 9% |
| Agricultural and Biological Sciences | 1 | 9% |
| Social Sciences | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 1 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2018.
All research outputs
#17,987,106
of 23,099,576 outputs
Outputs from Biogerontology
#484
of 661 outputs
Outputs of similar age
#238,054
of 331,031 outputs
Outputs of similar age from Biogerontology
#8
of 11 outputs
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