| Title |
Influence of media supplements on inhibition of oxidative browning and bacterial endophytes of Camellia sinensis var. sinensis
|
|---|---|
| Published in |
3 Biotech, August 2018
|
| DOI | 10.1007/s13205-018-1378-9 |
| Pubmed ID | |
| Authors |
Karthikeyan Alagarsamy, Lubobi Ferdinand Shamala, Shu Wei |
| Abstract |
Explant oxidative browning and necrosis of Camellia sinensis var. sinensis is a severe problem in tissue culture, often associated with the exuded phenolic compounds and microbial contamination from the explants. In this study, 2-aminoindane-2-phosphonic acid (AIP), an inhibitor of the polyphenol production-required enzyme phenylalanine ammonia lyase (PAL), and different antibiotics were tested to control tea explant necrosis and browning. These compounds were supplemented in the regular plant growth medium together with 6-benzylaminopurine and thidiazuron at different concentrations. Our data indicated that application of 2 µM of AIP was able to effectively inhibit callus browning, significantly reduce EGC abundance, and greatly improve callus induction and growth. Moreover, the use of 150 mg/L of timentin and 30 mg/L gentamycin resulted in an effective elimination of the surface and endophytic microbes associated with explants of C. sinensis var. sinensis. Our study revealed that the inhibition of PAL using AIP combined with the two tested antibiotics could open up new doors to control oxidative tissue browning and endophyte contamination in tissue culture for tea genetic manipulation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 15% |
| Student > Doctoral Student | 3 | 11% |
| Student > Ph. D. Student | 3 | 11% |
| Student > Master | 3 | 11% |
| Professor | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 11 | 41% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 37% |
| Chemical Engineering | 1 | 4% |
| Environmental Science | 1 | 4% |
| Unspecified | 1 | 4% |
| Business, Management and Accounting | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 12 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2018.
All research outputs
#18,646,262
of 23,100,534 outputs
Outputs from 3 Biotech
#646
of 1,252 outputs
Outputs of similar age
#254,592
of 331,125 outputs
Outputs of similar age from 3 Biotech
#22
of 50 outputs
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