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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Light-induced cell damage in live-cell super-resolution microscopy
|
|---|---|
| Published in |
Scientific Reports, October 2015
|
| DOI | 10.1038/srep15348 |
| Pubmed ID | |
| Authors |
Sina Wäldchen, Julian Lehmann, Teresa Klein, Sebastian van de Linde, Markus Sauer |
| Abstract |
Super-resolution microscopy can unravel previously hidden details of cellular structures but requires high irradiation intensities to use the limited photon budget efficiently. Such high photon densities are likely to induce cellular damage in live-cell experiments. We applied single-molecule localization microscopy conditions and tested the influence of irradiation intensity, illumination-mode, wavelength, light-dose, temperature and fluorescence labeling on the survival probability of different cell lines 20-24 hours after irradiation. In addition, we measured the microtubule growth speed after irradiation. The photo-sensitivity is dramatically increased at lower irradiation wavelength. We observed fixation, plasma membrane permeabilization and cytoskeleton destruction upon irradiation with shorter wavelengths. While cells stand light intensities of ~1 kW cm(-2) at 640 nm for several minutes, the maximum dose at 405 nm is only ~50 J cm(-2), emphasizing red fluorophores for live-cell localization microscopy. We also present strategies to minimize phototoxic factors and maximize the cells ability to cope with higher irradiation intensities. |
X Demographics
The data shown below were collected from the profiles of 51 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 11 | 22% |
| Japan | 8 | 16% |
| United Kingdom | 3 | 6% |
| China | 1 | 2% |
| Ireland | 1 | 2% |
| Germany | 1 | 2% |
| France | 1 | 2% |
| Unknown | 25 | 49% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 40 | 78% |
| Scientists | 9 | 18% |
| Science communicators (journalists, bloggers, editors) | 2 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 608 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 6 | <1% |
| United Kingdom | 3 | <1% |
| United States | 3 | <1% |
| Finland | 1 | <1% |
| Belgium | 1 | <1% |
| Netherlands | 1 | <1% |
| Unknown | 593 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 168 | 28% |
| Researcher | 94 | 15% |
| Student > Bachelor | 70 | 12% |
| Student > Master | 58 | 10% |
| Student > Doctoral Student | 24 | 4% |
| Other | 73 | 12% |
| Unknown | 121 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 106 | 17% |
| Physics and Astronomy | 102 | 17% |
| Agricultural and Biological Sciences | 94 | 15% |
| Engineering | 65 | 11% |
| Chemistry | 48 | 8% |
| Other | 70 | 12% |
| Unknown | 123 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 40. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2022.
All research outputs
#1,051,342
of 25,959,914 outputs
Outputs from Scientific Reports
#10,793
of 142,909 outputs
Outputs of similar age
#15,514
of 297,915 outputs
Outputs of similar age from Scientific Reports
#229
of 2,660 outputs
Altmetric has tracked 25,959,914 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 142,909 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.8. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 297,915 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 2,660 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.