Title |
Fractional deuteration applied to biomolecular solid-state NMR spectroscopy
|
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Published in |
Journal of Biomolecular NMR, November 2011
|
DOI | 10.1007/s10858-011-9585-2 |
Pubmed ID | |
Authors |
Deepak Nand, Abhishek Cukkemane, Stefan Becker, Marc Baldus |
Abstract |
Solid-state Nuclear Magnetic Resonance can provide detailed insight into structural and dynamical aspects of complex biomolecules. With increasing molecular size, advanced approaches for spectral simplification and the detection of medium to long-range contacts become of critical relevance. We have analyzed the protonation pattern of a membrane-embedded ion channel that was obtained from bacterial expression using protonated precursors and D(2)O medium. We find an overall reduction of 50% in protein protonation. High levels of deuteration at H(α) and H(β) positions reduce spectral congestion in ((1)H,(13)C,(15)N) correlation experiments and generate a transfer profile in longitudinal mixing schemes that can be tuned to specific resonance frequencies. At the same time, residual protons are predominantly found at amino-acid side-chain positions enhancing the prospects for obtaining side-chain resonance assignments and for detecting medium to long-range contacts. Fractional deuteration thus provides a powerful means to aid the structural analysis of complex biomolecules by solid-state NMR. |
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Geographical breakdown
Country | Count | As % |
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France | 1 | 2% |
Canada | 1 | 2% |
Unknown | 46 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 11 | 22% |
Researcher | 11 | 22% |
Professor > Associate Professor | 7 | 14% |
Student > Master | 5 | 10% |
Student > Bachelor | 2 | 4% |
Other | 6 | 12% |
Unknown | 7 | 14% |
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---|---|---|
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Physics and Astronomy | 2 | 4% |
Medicine and Dentistry | 1 | 2% |
Other | 1 | 2% |
Unknown | 9 | 18% |