Title |
A latent green fluorescent styrylcoumarin probe for the selective growth and detection of Gram negative bacteria
|
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Published in |
Bioorganic & Medicinal Chemistry, August 2018
|
DOI | 10.1016/j.bmc.2018.08.015 |
Pubmed ID | |
Authors |
Linda Váradi, Miaoyi Wang, Ramesh R Mamidi, Jia Lin Luo, John D Perry, David E Hibbs, Paul W Groundwater |
Abstract |
A novel, green fluorescent β-alanylstyrylcoumarin derivative was synthesized and evaluated for its performance as a fluorogenic enzyme substrate on a range of clinically relevant microorganisms. The substrate was selectively hydrolysed by β-alanyl aminopeptidase producing P. aeruginosa resulting in an on-to-off fluorescent signal. Growth inhibitory effect of the substrate was observed on Gram positive bacteria and yeasts. Meanwhile, Gram negative species, despite their extremely protective cell envelope, showed ready uptake and accumulation of the substrate within their healthy growing colonies displaying intense green fluorescence. |
X Demographics
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United Kingdom | 4 | 57% |
Australia | 1 | 14% |
Unknown | 2 | 29% |
Demographic breakdown
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Members of the public | 4 | 57% |
Scientists | 3 | 43% |
Mendeley readers
Geographical breakdown
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 21% |
Student > Ph. D. Student | 3 | 21% |
Other | 2 | 14% |
Lecturer | 1 | 7% |
Lecturer > Senior Lecturer | 1 | 7% |
Other | 3 | 21% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
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Chemistry | 8 | 57% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Business, Management and Accounting | 1 | 7% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Other | 1 | 7% |
Unknown | 1 | 7% |