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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Proteomic analysis of exosomes reveals an association between cell invasiveness and exosomal bioactivity on endothelial and mesenchymal cell migration in vitro.
|
|---|---|
| Published in |
Clinical Science, September 2018
|
| DOI | 10.1042/cs20180425 |
| Pubmed ID | |
| Authors |
Shayna Sharma, Mona Alharbi, Miharu Kobayashi, Andrew Lai, Dominic Guanzon, Felipe Zuñiga, Valeska Ormazabal, Carlos Palma, Katherin Scholz-Romero, Gregory E Rice, John D Hooper, Carlos Salomon |
| Abstract |
Ovarian cancer has resulted in over 140,000 deaths reported annually, worldwide. This is often attributed to cellular changes in the microenvironment, including increased migration of mesenchymal stem cells (MSCs) and endothelial cells (ECs) to facilitate metastasis. Recently, the ability of exosomes to communicate signals between cells (and promote cancer progression) has been established. In this study, we explored the effect of exosomes on cells present in the tumour microenvironment. Exosomes were isolated from ovarian cancer cells with different invasive capacity (high=SKOV-3 and low=OVCAR-3) by differential and buoyant density centrifugation and characterized using nanoparticle tracking analysis, western blot and electron microscopy. Exosome secretion was positively correlated with invasiveness of releasing cells. Proteomic analyses identified common and unique proteins between exosomes from SKOV-3 and OVCAR-3 with gene ontology analyses revealing that these exosomes are involved in the regulation of cell migration. Since the tumour microenvironment contains multiple cell types, including mesenchymal stem cells and endothelial cells, we examined the effect of these exosomes on MSC and EC migration. Exosomes promoted MSC and EC migration in a time- and concentration-dependent manner. The effect of exosomes isolated from SKOV-3 on cell migration was significantly higher compared to exosomes from OVCAR-3. Thus, we suggest that exosomes from ovarian cancer cells contain a specific set of proteins that are representative of its cell of origin and the invasive capacity. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Korea, Republic of | 1 | 33% |
| Australia | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 21% |
| Researcher | 5 | 15% |
| Student > Bachelor | 5 | 15% |
| Student > Doctoral Student | 4 | 12% |
| Student > Master | 4 | 12% |
| Other | 2 | 6% |
| Unknown | 7 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 35% |
| Medicine and Dentistry | 8 | 24% |
| Agricultural and Biological Sciences | 2 | 6% |
| Engineering | 2 | 6% |
| Social Sciences | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 8 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 May 2020.
All research outputs
#13,107,306
of 23,100,534 outputs
Outputs from Clinical Science
#1,426
of 2,329 outputs
Outputs of similar age
#162,812
of 341,986 outputs
Outputs of similar age from Clinical Science
#9
of 28 outputs
Altmetric has tracked 23,100,534 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,329 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,986 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.