| Title |
Characterization of the binding sites of the anticancer ruthenium(III) complexes KP1019 and KP1339 on human serum albumin via competition studies
|
|---|---|
| Published in |
JBIC Journal of Biological Inorganic Chemistry, October 2012
|
| DOI | 10.1007/s00775-012-0944-6 |
| Pubmed ID | |
| Authors |
Orsolya Dömötör, Christian G. Hartinger, Anna K. Bytzek, Tamás Kiss, Bernhard K. Keppler, Eva A. Enyedy |
| Abstract |
Indazolium trans-[tetrachloridobis(1H-indazole)ruthenate(III)] (KP1019) and its Na(+) analogue (KP1339) are two of the most prominent non-platinum antitumor metal complexes currently undergoing clinical trials. After intravenous administration, they are known to bind to human serum albumin (HSA) in a noncovalent manner. To elucidate their HSA binding sites, displacement reactions with the established site markers warfarin and dansylglycine as well as bilirubin were monitored by spectrofluorimetry, ultrafiltration-UV-vis spectrophotometry, and/or capillary zone electrophoresis. Conditional stability constants for the binding of KP1019 and KP1339 to sites I and II of HSA were determined, indicating that both Ru(III) compounds bind to both sites with moderately strong affinity (log K(1)' = 5.3-5.8). No preference for either binding site was found, and similar results were obtained for both metal complexes, demonstrating low influence of the counter ion on the binding event. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Italy | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 69 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 18% |
| Student > Bachelor | 11 | 15% |
| Researcher | 10 | 14% |
| Student > Master | 5 | 7% |
| Professor > Associate Professor | 4 | 6% |
| Other | 10 | 14% |
| Unknown | 19 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 38 | 53% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Agricultural and Biological Sciences | 4 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Medicine and Dentistry | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 22 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 October 2015.
All research outputs
#7,331,154
of 23,842,189 outputs
Outputs from JBIC Journal of Biological Inorganic Chemistry
#172
of 664 outputs
Outputs of similar age
#53,058
of 177,711 outputs
Outputs of similar age from JBIC Journal of Biological Inorganic Chemistry
#2
of 6 outputs
Altmetric has tracked 23,842,189 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 664 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 177,711 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.